Abstract
We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections.
MeSH terms
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Adult
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Aged
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Antibodies, Viral / immunology*
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Antigens, Viral / immunology
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Cross Reactions
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Dengue / blood
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Dengue / immunology
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Dengue Virus / immunology
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Female
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Humans
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Immunoglobulin A / immunology*
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Immunoglobulin G / immunology*
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Male
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Middle Aged
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Nanotechnology*
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RNA, Viral / blood
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Reverse Transcriptase Polymerase Chain Reaction
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Viral Nonstructural Proteins / immunology*
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Young Adult
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Zika Virus / immunology
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Zika Virus Infection / blood
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Zika Virus Infection / diagnosis*
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Zika Virus Infection / immunology
Substances
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Antibodies, Viral
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Antigens, Viral
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Immunoglobulin A
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Immunoglobulin G
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RNA, Viral
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Viral Nonstructural Proteins