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The subcellular localization of administered N-acetylneuraminic acid in the brains of well-fed and protein restricted rats

Published online by Cambridge University Press:  09 March 2007

Brian L. G. Morgan  and
Myron Winick
Brian L. G. Morgan
Affiliation:
Institute of Human Nutrition, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Myron Winick
Affiliation:
Institute of Human Nutrition, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
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Abstract

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1. This study investigated the subcellular localization of injected N-acetylneuraminic acid (NeuNAc) in brain. Forty pregnant rats were distributed into four groups. Two groups were given a 200 g casein/kg diel and the other two groups a 100 g casein/kg diet throughout gestation. One group from each of the low- and high-protein groups were given their respective diets for the first 11 d of lactation. On day 12 of lactation, 2·5 μCi [14]NeuNAc/kg body-weight were injected intraperitoneally into their pups. After 1 h the pups were killed, their brains removed and subjected to subcellular fractionation. On day 16 of lactation the other two groups were similarly treated.

2. In all groups of animals 80% of the [14C]NeuNAc incorporated into the brains was found in the synaptosomalfraction and the remainder distributed among the other subcellular fractions in proportion to their total NeuNA ccontent.

3. These results suggest that NeuNAc exerts its effects on behaviour via the synaptic membrane.

Type
Papers of direct reference to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 46 , Issue 2 , September 1981 , pp. 231 - 238
Copyright
Copyright © The Nutrition Society 1981

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