Abstract
The protein Sonic hedgehog (Shh) controls patterning and growth during vertebrate development. Here we demonstrate that it binds Patched (vPtc), which has been identified as a tumour-suppressor protein in basal cell carcinoma, with high affinity. We show that Re can form a physical complex with a newly cloned vertebrate homologue of the Drosophila protein Smoothened (vSmo), and that vSmo is coexpressed with vPtc in many tissues but does not bind Shh directly. These findings, combined with available genetic evidence from Drosophila, support the hypothesis that Ptc is a receptor for Shh, and that vSmo could be a signalling component that is linked to Ptc.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Echelard, Y. et al. Cell 75, 1417–1430 (1993).
Krauss, S., Concordet, J.-P. & Ingham, P. W. Cell 75, 1431–1444 (1993).
Riddle, R. D., Johnson, R. L., Laufer, E. & Tabin, C. Cell 75, 1401–1416 (1993).
Roelink, H. et al. Cell 76, 761–775 (1994).
Currie, P. D. & Ingham, P. W. Nature 382, 452–455 (1996).
Ekker, S. C. et al. Curr. Biol. 5, 944–955 (1995).
Roelink, H. et al. Cell 81, 445–455 (1995).
Marti, E., Bumcrot, D. A., Takada, R. & McMahon, A. P. Nature 375, 322–324 (1995).
Yamada, T., Placzek, M., Tanaka, H., Dodd, J. & Jessell, T. M. Cell 64, 635–647 (1991).
Hynes, M., Poulsen, K., Tessier-Lavigne, M. & Rosenthal, A. Cell 80, 95–101 (1995).
Ericson, J., Muhr, J., Jessel, J. & Edlund, T. Int. J. Dev. Biol. 39, 809–816 (1995).
Fan, C.-M. et al. Cell 81, 457–465 (1995).
Johnson, R. L., Laufer, E., Riddle, R. D. & Tabin, C. Cell 79, 1165–1173 (1994).
Niswander, L., Jeffrey, S., Martin, G. R. & Tickle, C. Nature 371, 609–612 (1994).
Laufer, E., Nelson, C. E., Johnson, R. L., Morgan, B. A. & Tabin, C. Cell 79, 993–1003 (1994).
Roberts, D. J. et al. Development 121, 3163–3174 (1995).
Chiang, C. et al. Nature 382, 407–413 (1996).
Bitgood, M. J., Shen, L. & McMahon, A. P. Curr. Biol. 6, 298–304 (1996).
Vortkamp, A. et al. Science 273, 613–622 (1996).
Hooper, J. E. & Scott, M. P. Cell 59, 751–765 (1989).
Nakano, Y. et al. Nature 341, 508–513 (1989).
Hidalgo, A. & Ingham, P. Development 110, 291–301 (1990).
Ingham, P. W., Taylor, A. M. & Nakano, Y. Nature 353, 184–187 (1991).
Nüsslein-Volhard, C., Wieschaus, E. & Kluding, H. Wilhelm Roux Arch. Dev. Biol. 193, 267–282 (1984).
Jürgens, G., Wieschaus, E., Nüsslein-Volhard, C. & Kluding, H. Wilhelm Roux Arch. Dev. Biol. 193, 283–295 (1984).
Alcedo, J., Ayzenzon, M., Van Ohlen, T., Noll, M. & Hooper, J. E. Cell 86, 221–232 (1996).
van den Heuvel, M. & Ingham, P. W. Nature 382, 547–551 (1996).
Marigo, V., Scott, M. P., Johnson, R. L., Goodrich, L. V. & Tabin, C. J. Development 122, 1225–1233 (1996).
Goodrich, L. V., Johnson, R. L., Milenkovic, L., McMahon, J. A. & Scott, M. P. Genes Dev. 10, 301–312 (1996).
Hahn, H. et al. Cell 85, 841–851 (1996).
Johnson, R. L. et al. Science 272, 1668–1671 (1996).
Bhanot, P. et al. Nature 382, 225–230 (1996).
Chan, S. D. H. et al. J. Biol. Chem. 267, 25202–25207 (1992).
Strader, C. D., Fong, T. M., Tota, M. R. & Underwood, D. Annu. Rev. Biochem. 63, 101–132 (1994).
Lee, J. J. et al. Science 266, 1528–1537 (1994).
Middlemas, D. S., Lindberg, R. A. & Hunter, T. Mol. Cell. Biol. 11, 143–153 (1991).
Holmes, W. E., Lee, J., Kunag, W. J., Rice, G. C. & Wood, W. I. Science 253, 1278–1280 (1991).
Davis, S. et al. Science 259, 1736–1739 (1993).
Treanor, J. J. S. et al. Nature 382, 80–83 (1996).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stone, D., Hynes, M., Armanini, M. et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog. Nature 384, 129–134 (1996). https://doi.org/10.1038/384129a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/384129a0
This article is cited by
-
Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1
Nature Communications (2023)
-
Sonic Hedgehog Signaling Pathway: A Role in Pain Processing
Neurochemical Research (2023)
-
Evaluation of Expression of WNT1 and PTCH Genes in Peripheral Blood of Patients with Odontogenic Cysts and Tumours of the Jaws by Quantitative RT-PCR: A Pilot Study
Journal of Maxillofacial and Oral Surgery (2023)
-
The role of Smo-Shh/Gli signaling activation in the prevention of neurological and ageing disorders
Biogerontology (2023)
-
Hedgehog-mediated gut-taste neuron axis controls sweet perception in Drosophila
Nature Communications (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.