TY - JOUR T1 - No association between abdominal pain and <em>Dientamoeba</em> in Dutch and Belgian children JF - Archives of Disease in Childhood JO - Arch Dis Child SP - 686 LP - 689 DO - 10.1136/archdischild-2018-316383 VL - 104 IS - 7 AU - Martijn Ramon Brands AU - Els Van de Vijver AU - Sjoukje Marije Haisma AU - Anke Heida AU - Patrick Ferry van Rheenen Y1 - 2019/07/01 UR - http://adc.bmj.com/content/104/7/686.abstract N2 - Objective To study the association between Dientamoebafragilis colonisation and faecal calprotectin to see whether the parasite is a harmless commensal or a gut pathogen.Design Cross-sectional study of previously collected stool samples.Setting and patients Two hundred stool samples originated from children aged 5–19 years with chronic abdominal pain and diarrhoea, who were seen in paediatric clinics in the Netherlands and Belgium and in whom somatic gastrointestinal disorders were excluded. Another 122 samples came from a healthy community-based reference population of the same age. All stool samples were analysed with real-time PCR for the detection of D. fragilis and with an ELISA for calprotectin—a biomarker of gastrointestinal inflammation.Main outcome measures Prevalence of D. fragilis colonisation and results of stool calprotectin testing.Results D. fragilis was detected in 45% (95% CI 38% to 51%) of patients and in 71% (95% CI 63% to 79%) of healthy children. Median (IQR) concentrations of calprotectin in patients and healthy children with a positive PCR result were not different from those with a negative PCR result (40 (40–55) μg/g vs 40 (40–75) μg/g, respectively).Conclusion Since D. fragilis colonisation is most prevalent in healthy children and is not associated with an increase in faecal calprotectin concentration, our data do not support the inference that D. fragilis is a pathogenic parasite. Routinely testing for D. fragilis in children with chronic abdominal pain should therefore be discouraged. ER -