PT  - JOURNAL ARTICLE
AU  - Zeeba Zaka-ur-Rab
AU  - Shaad Abqari
AU  - Tabassum Shahab
AU  - Najmul Islam
AU  - Indu Shukla
TI  - Evaluation of salivary anti-<em>Salmonella typhi</em> lipopolysaccharide IgA ELISA for serodiagnosis of typhoid fever in children
AID  - 10.1136/adc.2011.300622
DP  - 2012 Mar 01
TA  - Archives of Disease in Childhood
PG  - 236--238
VI  - 97
IP  - 3
4099  - http://adc.bmj.com/content/97/3/236.short
4100  - http://adc.bmj.com/content/97/3/236.full
SO  - Arch Dis Child2012 Mar 01; 97
AB  - This observational study was conducted to determine the diagnostic accuracy of ELISA for the detection of anti-Salmonella typhi lipopolysaccharide (LPS) salivary immunoglobulin A (IgA) antibodies in 37 children with culture confirmed typhoid, 30 febrile controls with an alternative diagnosis and 30 healthy controls. The test was positive in 33/37 (89.2%) cases of typhoid, but negative in all patients in the two control groups. Maximum absorbance of anti-LPS IgA was observed during the second and third weeks of typhoid, with a progressive decline thereafter. The sensitivity of ELISA was 71.4%, 100%, 100%, 9.1% and 0%, in first, second, third, fourth and fifth week of illness, respectively. Further large scale studies measuring salivary anti-LPS IgA antibodies are needed to confirm the potential of saliva-based serology in children with suspected typhoid.