Background and objective Frequency and clinical manifestations of congenital toxoplasmosis in Iran is not determined, object of study was to determine the Frequency of positive serologic neonates for Toxoplasma from birth and follow up of them.

Methods In a cohort prospective study (2011–2012), Cord blood sample obtained from 270 neonates, toxoplasma serology tests (IgG, IgM) done, cases with positive toxo-IgM treated and followed

Finding Positive IgM and IgG determined 1.5%, 44.1% respectively. The most common manifestation was Eye (50%) and brain (50%).

Conclusion Early treatment of infected neonates and wide variation of toxoplasma infection in country is so important. Adding the toxoplasma serologic tests to neonatal screening test is needed and recommended.