Article Text

Download PDFPDF

PS-240 Developing A Toxic Paediatric Animal Model Of Non-oliguric Acute Renal Injury With Cisplatin
  1. MJ Santiago,
  2. J López-Herce,
  3. J López,
  4. J Urbano,
  5. R González,
  6. MJ Solana,
  7. SN Fernandez,
  8. B Toledo
  1. PICU, Hospital General Universitario Gregorio Marañón, Madrid, Spain


Introduction Developing a non-oliguric paediatric animal model of acuterenal injury (AKI) could be useful to study the evolution of diuresis after treatments. Cisplatin causes a dose-dependant poliuric renal failure in humans. A dose of 5 mg/kg has been used in rats to produced AKI but there are no studies in pigs.

Objectives To define the target dose of Cisplatin that develops anon-oliguric toxic acute kidney injury in piglets.

Methods A prospective experimental study was performed in 8 piglets (mean 10 kg). Three different intravenous doses of Cisplatin (2, 3 and5 mg/kg) and two different periods of time between administration and evaluation (2 and 4 days) were studied. Urine and blood samples were collected.

Results Results are presented in Table 1. A dose of 2 mg/kg did not produce important alteration of renal function at any given time. A very severe oliguric AKI with extremely high hyperkalemia was observed four days after a 3 mg/kg dose and 3 days after a 5 mg/kd dose. A dose of 3 mg/kg administrated 48 h before produced an important AKI without severe hyperkalemia.

Abstract PS-240 Table 1

Conclusions A dose of 3 mg/kgof intravenous cisplatin producednon-oliguric AKI after 48 h in piglets. This dose and interval can be used for toxic paediatric animal models of AKI.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.