Background and Aims Preterm infants need effective pain management during and after the frequent painful procedures that guarantee their survival, whereas insufficient pain control may have negative impact on development of cognition, motor function and behaviour in preterm infants. The aim of this study was to assess the analgesic effect of lullaby music on pain score during venipuncture in preterm infants.

Methods 60 healthy preterm infants with a gestational age of 32–36 weeks randomly assigned to two groups in a double blind manner (30 in each group). For infants in experimental group lullaby music, with 40–50 db, was played 2 min before venipuncture. In control group, infants did not receive any intervention. For two groups, Two video systems were used to videotape the infant's face and monitor during study. pain scores were calculated by using Premature Infant Pain Profile (PIPP) before intervention, during the 30 sec after needle insertion, 30 sec after removal needle and 10^th min after the end of venipuncture.

Results During the 30 sec after needle insertion, there was no significant decrease in pain score in experimental group to compare with control group (p=0.075) whereas 30 sec after the end of venipuncture, reduction in pain score was observed in experimental group (p=0.0001).

Conclusion In lullaby music group, reduction in pain score during venipuncure did not observe, but Immediately after the end of procedure, significantly pain score decreased. So duration of playing music can be affected on pain responses during venipuncture in preterm infants.