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1529 The Study of the Effect of Cumin Seeds Extracts on some Pathogenic Bacterial Agents
  1. M Motamedifar1,
  2. G Haghighati2,
  3. A Yousefi Arvand2
  1. 1Department of Bacteriology and Virology, Shiraz AIDS Research Center (SHARC), Shiraz University of Medical Sciences
  2. 2Department of Bacteriology and Virology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran


Background and Aims The antimicrobial effects of cumin (Cuminum cyminum L.), as an agent, have been mentioned previously. In this study the inhibitory effect of alcoholic and aqueous extracts on some Gram positive and Gram negative pathogenic bacteria was examined.

Methods Susceptibility of different standard bacterial strains to the prepared methanolic and aqueous extracts were studied by using disk diffusion method in comparison with penicillin and amikacin antibiotics. The bacteria studied were Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Kelebsiella pneumoniae (ATCC 10031), Bacillus cereus (ATCC 11778), meticillin susceptible Staphylococcus aureus (MSSA) ATCC 25923 and meticillin resistant Staphylococcus aureus (MRSA) ATCC 700698. The minimum inhibitory concentration (MIC) of extracts was also determined when necessary.

Results The Methanolic and aqueous extracts of Cuminum cyminum (25–100 mg/mL) caused growth inhibition zones of S. aureus with the diameter ranged ranging from 9.6–22.4 mm and 14–23.2 mm respectively. For MRSA growth inhibition zones ranged 13–23.4 mm for alcoholic extracts and 11–13.2 mm for the aqueous extract. For MSSA Staphylococci the methanolic extract caused inhibition diameter of 13 mm and aqueous extract with the diameter of 10–13 mm. These extracts were ineffective on other investigated bacteria. MIC of alcoholic extracts for MRSA (700698) and MSSA (25923) was determined as 75±35.36 mg/mL.

Conclusions Considering the antibacterial effects of alcoholic and aqueous extracts of cumin seeds on S. aureus observed in this investigation, continuing the research for studying the in-vivo effect of ant- microbial effects of Cunminum cyminum extracts on Staphylococci seems valuable.

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