Objective Invasive painful procedures are among the neonate’s medical care. Among them venipuncture for bilirubin check is the most prevalent one. Pain has some early and late onset effects in neonates. Now a day there is a great interest in analgesic treatment in neonates. Several comfort measures and medications have been used to alleviate procedural pain in neonates. This study was carried out to assess the analgesic effect of 30% oral glucose solution in pain of venipuncture.

Methods Sixty term neonates admitted for phototherapy because of hyperbilirubinemia randomized in to two groups. 30 neonates as a case received 2 cc of 30% oral glucose solution and 30 another neonates received 2 cc of distilled water, 2 minutes before venipuncture. Prechtl’s score was used to assess the arousal state before procedure and Douler aigueolu nouveau-ne (DAN) score was used to assess behavioral response to pain within 30 seconds after venipuncture. Pain score compared between two groups and a p-value less than 0.05 considered being significant.

Results The arousal state score before venipuncture was the same in two groups (mean prechtl’s score 2.6 for distilled water and 2.3 for 30% glucose (p = 0.265) that showed no significant difference. But mean DAN score was 8.1 for distilled water and 4.2 for glucose that showed a significant difference in pain due to venipuncture between two groups (p = 0.000).

Conclusion 30% oral glucose solution does reduce the neonatal pain and can be used safely before minor procedures.