Introduction It is unrealistic to hope for a germ free NICU, but it is possible to reduce the likelihood of poor hygiene. NICU hygiene is affected drastically by the medical staff and the internal environment. This study will describe some of interactions between the laboratory and improper housekeeping procedures.

Methods The present study was conducted in the NICU of Damanhour Teaching Hospital. Cleanliness and sanitation was evaluated by bacterioloical examination of the umbilical stump and the internal enviroment of the wards represented by floor, air, baby balance, baby heat, air conditioning devices and baby care units.

Results The study revealed the failure of the routine housekeeping process. The study suggested 3 categories to control and prevent NICU nosocomial infections. Also suggested is a technique for cleaning and sanitation of ward baby incubators by using stabilised hydrogen peroxide 6% as a disinfectant after cleaning by natural soap and not using any toxic or irritant materials.

The technic succeeded 100% in the removal of all pathogenic microorganisms; using stabilised hydrogen peroxide is the safest disinfectant instead of other toxic disinfectants.

Conclusion The study revealed failures of routine housekeeping processes, and suggests 3 methods to control and prevent NICU nosocomial infections. It also proposed a new technique, for cleaning wards and baby incubators, and assures a result of 100% in removing pathogenic microorganisms.