Article Text

Download PDFPDF

Systemic neutrophil activation in acute preschool viral wheeze
  1. A Oommen1,
  2. R Patel2,
  3. M Browning2,
  4. J Grigg1
  1. 1Leicester Children’s Asthma Centre, Institute for Lung Health, University of Leicester, PO Box 65, Leicester LE2 7LX, UK
  2. 2Department of Immunology, Leicester Royal Infirmary, University Hospitals of Leicester, Infirmary Square, Leicester LE1 5WW, UK
  1. Correspondence to:
    Dr J Grigg, Senior Lecturer in Paediatric Respiratory Medicine, Leicester Children’s Asthma Centre, Institute for Lung Health, University of Leicester, Leicester LE2 7LX, UK;


Background: In preschool children, attacks of wheeze are usually triggered by viral colds. The inflammatory substrate in preschool viral wheeze (PVW) is unclear, but epidemiological data suggest that most PVW is not caused by allergic inflammation. We therefore speculated that the neutrophils are an important effector cell. Systemic neutrophil activation is the first stage for the development of pulmonary neutrophilia. Markers of neutrophil activation are shedding of the adhesion molecule L-selectin from the cell surface, upregulation of Mac-1 expression, and an increase in serum soluble L-selectin.

Aims: To obtain evidence for systemic neutrophil activation during PVW.

Methods: Preschool children (1–5 years) admitted to hospital with acute PVW (n = 20) and normal controls (n = 18) were studied. Adhesion molecule expression on CD16 positive neutrophils was determined in both groups and expressed as molecules of equivalent fluorochrome (MEF). Serum soluble L-selectin was analysed by ELISA.

Results: Compared with controls, children with PVW had reduced neutrophil L-selectin expression (median MEF (IQR): 69 (11 to 96) units versus 136 (109 to 163) units, p < 0.001) and higher serum soluble L-selectin (2.8 (2.3 to 3.1) versus 2.4 (2.2 to 2.6) μg/ml, p = 0.04). There was no significant difference in neutrophil Mac-1 expression.

Conclusion: Systemic neutrophil activation is associated with acute PVW.

  • L-selectin
  • preschool
  • neutrophil
  • viral wheeze
  • BAL, bronchoalveolar lavage
  • IQR, interquartile range
  • MEF, molecules of equivalent fluorochrome
  • PVW, preschool viral wheeze
View Full Text

Statistics from


    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.