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Acute paediatrics
Diagnosis of group A coxsackieviral infection using polymerase chain reaction
  1. M Hosoya1,
  2. H Ishiko2,
  3. Y Shimada2,
  4. K Honzumi1,
  5. S Suzuki3,
  6. K Kato3,
  7. H Suzuki1
  1. 1Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
  2. 2Infectious Disease Laboratory, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo 174-8555, Japan
  3. 3Fukushima Institute for Public Health and Environmental Science, Fukushima 960-8560, Japan
  1. Correspondence to:
    Dr M Hosoya, Dept of Pediatrics, Fukushima Medical University School of Medicine, Hikarigaoka 1, Fukushima 960-1295, Japan;
    mhosoya{at}fmu.ac.jp

Abstract

Aims: To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR).

Methods: Throat swabs were collected from 246 children from June to August 1997 and 1998.

Results: Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Enteroviral genomes were detected in 54/178 samples from which no virus was isolated. Of 41 enteroviral genotypes identified by sequence analysis of PCR products, 38 were group A coxsackieviruses, which are usually difficult to isolate using tissue culture.

Conclusion: Results indicate that viral detection and identification based on PCR is useful in the diagnosis of group A coxsackieviral infection.

  • coxsackievirus group A
  • herpangina
  • polymerase chain reaction
  • phylogenetic

https://doi.org/10.1136/adc.87.4.316

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