Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine

I Claassen; J Meylis; P van der Ley; C Peeters; H Brons; J Robert; D Borsboom; A van der Ark; I van Straaten; P Roholl; B Kuipers; J Poolman

doi:10.1016/0264-410x(96)00020-5

Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine

Vaccine. 1996 Jul;14(10):1001-8. doi: 10.1016/0264-410x(96)00020-5.

Authors

I Claassen¹, J Meylis, P van der Ley, C Peeters, H Brons, J Robert, D Borsboom, A van der Ark, I van Straaten, P Roholl, B Kuipers, J Poolman

Affiliation

¹ Laboratory for the Control of Biological Products, National Institute for Public Health and Environmental Protection, Bilthoven, The Netherlands.

PMID: 8873395
DOI: 10.1016/0264-410x(96)00020-5

Abstract

An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16;P1.5,2;P1.19,15 and P1.5c,10;P1.12,13;P1.7h,4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various por A genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Outer Membrane Proteins / immunology*
Bacterial Vaccines / immunology*
Female
Humans
Meningitis, Meningococcal / prevention & control
Mice
Microscopy, Electron
Neisseria meningitidis / immunology*

Substances

Bacterial Outer Membrane Proteins
Bacterial Vaccines