Typing and subtyping clinical isolates of influenza virus using reverse transcription-polymerase chain reaction

doi:10.1016/S0928-0197(96)00254-1

Clinical and Diagnostic Virology

Volume 7, Issue 2, November 1996, Pages 77-84

Clinical and Diagnos...

https://doi.org/10.1016/S0928-0197(96)00254-1 Get rights and content

Abstract

Background: Influenza virus infections are a major cause of morbidity and the identification of the type or subtype of a clinical isolate has important clinical and epidemiological implications.

Objectives: To evaluate the ability of a reverse transcription-polymerase chain reaction (RT-PCR) assay to type and subtype clinical human isolates of influenza virus.

Study design: Reference strains of influenza $A H1N1$ , $A H3N2$ , and B viruses and human clinical isolates of influenza virus representing antigenic variants from the last 15 years were evaluated using an RT-PCR assay.

Results: Amplicons of 325, 198 and 365 base pairs in length were obtained from RNA extracted from influenza $A H1N1$ , $A H3N2$ and B viruses, respectively. All human-derived $A H1N1$ , $A H3N2$ , and B reference strains and antigenic variants tested were correctly identified.

Conclusions: RT-PCR is an effective alternative to traditional methods for typing and subtyping influenza viruses.

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ReportTyping and subtyping clinical isolates of influenza virus using reverse transcription-polymerase chain reaction

Abstract

Anal. Biochem.

J. Virol. Methods

Virus Res.

J. Virol. Methods

Sequence relationships among the hemagglutinin genes of 12 subtypes of influenza A virus

Classification of respiratory tract picornavirus isolates as enteroviruses or rhinoviruses by using reverse transcription-polymerase chain reaction

J. Clin. Microbiol.

Detection of enteric viruses in oysters using the polymerase chain reaction

Appl. Environ. Microbiol.

Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR

Appl. Environ. Microbiol.

Maintenance of viability and comparison of identification methods for influenza and other respiratory viruses of humans

J. Clin. Microbiol.

Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus

J. Clin. Microbiol.

Prospective application of reverse transcriptase polymerase chain reaction for diagnosing influenza infections in respiratory samples from a children's hospital

J. Clin. Microbiol.

Influenza: its control in persons and populations

J. Infect. Dis.

Report
Typing and subtyping clinical isolates of influenza virus using reverse transcription-polymerase chain reaction