Determination of plasma catecholamines by high performance liquid chromatography with electrochemical detection: Comparison with a radioenzymatic method
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Cited by (549)
Differential effects of enalapril-felodipine versus enalapril-lercanidipine combination drug treatment on sympathetic nerve traffic and metabolic profile in obesity-related hypertension
2016, Journal of the American Society of HypertensionCitation Excerpt :Measurements included BMI, waist-to-hip ratio, sphygmomanometric, beat-to-beat finger (Finapres; Ohmeda 2300, Englewood, Florida, USA) systolic BP, diastolic BP, heart rate (HR; electrocardiogram), respiration rate (pneumotachograph), and echocardiographically detected left ventricular mass index, calculated by the Devereux formulae normalized to body surface area.11,12 They also included multiunit recordings of muscle sympathetic nerve traffic (MSNA) via the microneurographic technique, as previously described,2,7,13 venous plasma norepinephrine (NE) measured by high-performance liquid chromatography,14 and fasting plasma glucose and plasma insulin, which were determined from a blood sample taken from an antecubital vein. From a standard formulae (plasma insulin × fasting plasma glucose/22.5), calculation was made of the homeostasis model assessment (HOMA) of insulin resistance, which was used as an estimate of insulin resistance.15
A 14-year follow-up study of chest pain patients including stress hormones and mental stress at index event
2012, International Journal of CardiologyCitation Excerpt :The samples were cold centrifuged (3000 rpm) at + 4° C and frozen at − 70 °C until analysis. High performance liquid chromatography (HPLC) with electrochemical detection was used for analysis [19]. The coefficients of variation (CV) for low and high values were for noradrenalin 8.8% and 9.8%, for adrenalin 10.1% and 9.5% and for dopamine 17.9% and 22.6%.
Acute altitude-induced hypoxia suppresses plasma glucose and leptin in healthy humans
2010, Metabolism: Clinical and ExperimentalCitation Excerpt :Blood for epinephrine determination was dispensed into prechilled tubes containing ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid and reduced glutathione. Epinephrine was determined by high-performance liquid chromatography (Waters Associates, Milford, MA) using electrochromatic detection as previously described [28,29]. Samples for lactate were collected in prechilled tubes containing 1 mL of 0.7 mol/L perchloric acid, in which 0.5 mL of whole blood was dispensed and mixed.
Differential sympathetic activation in muscle and skin neural districts in the metabolic syndrome
2009, Metabolism: Clinical and ExperimentalCitation Excerpt :Body mass index was obtained by dividing body weight in kilograms by the square of the height in meters. Plasma norepinephrine was measured by high-performance liquid chromatography [17] from a venous blood sample. The same blood sample was also used to assess the various metabolic variables useful for the diagnosis of metabolic syndrome (glycemia, triglycerides, total and high-density lipoprotein cholesterol) as well as routine hematologic and blood chemistry data (hemoglobin, blood urea, electrolytes, blood creatinine, etc).
Reproducibility patterns of plasma norepinephrine and muscle sympathetic nerve traffic in human obesity
2009, Nutrition, Metabolism and Cardiovascular DiseasesCitation Excerpt :This quantification has been shown to provide reproducible values that differ only by 3.8% when assessed twice in the same session by a single investigator [18]. Plasma norepinephrine was measured by high performance liquid chromatography [19] from a blood sample taken from an antecubital vein. The method enables the detection of norepinephrine changes of 5 pg/ml and the mean coefficient of variation of values obtained from the same sample was 5.4%.
Sympathetic activation in congestive heart failure: Reproducibility of neuroadrenergic markers
2008, European Journal of Heart Failure