Automation of 1,9-dimethylmethylene blue dye-binding assay for sulfated glycosaminoglycans with application to cartilage microcultures
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Cited by (23)
Glycosaminoglycans detection methods: Applications of mass spectrometry
2017, Molecular Genetics and MetabolismCitation Excerpt :In 1982, Farndale et al. overcame these limitations by substituting formate for the citrate/phosphate buffer used in the original assay [81]. Sabiston et al. developed an automated method [84], and Panin et al. adapted the method to use DMMB to measure GAGs in cetylpyridinium chloride precipitated urine [85]. Chandrasekhar et al. used DMMB to measure GAGs after chromatographic elution with guanidium chloride (GuHCl) [86] and Whitley et al. refined the method to measure GAGs in small amounts of sample [37].
Cooperation of binding sites at the hydrophilic domain of cell-surface sulfatase Sulf1 allows for dynamic interaction of the enzyme with its substrate heparan sulfate
2013, Biochimica et Biophysica Acta - General SubjectsCitation Excerpt :All the different GAGs had been immobilized in sufficient amounts as could be proven by flushing the columns with solutions of the sulfated GAG-specific dye dimethylmethylene Blue (DMMB) and recording the retention volumes needed to detect the dye in the eluate (Fig. S1A and B). Although DMMB binding capacities largely differed between the columns and a direct comparison of the immobilization degree is difficult due to GAG-type specific binding of DMMB [33], the amounts of immobilized GAG were sufficient to detect bound HD by Western blotting in the eluate of any column. To control for unspecific interactions with the matrix a column reacted with ethanolamine was generated.
Optimization of an alcian blue dot-blot assay for the detection of glycosaminoglycans and proteoglycans
1991, Analytical BiochemistryIsolation and Characterization of Proteoglycans
1987, Methods in EnzymologyImproved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue
1986, BBA - General Subjects