Elsevier

Analytical Biochemistry

Volume 149, Issue 2, September 1985, Pages 543-548
Analytical Biochemistry

Automation of 1,9-dimethylmethylene blue dye-binding assay for sulfated glycosaminoglycans with application to cartilage microcultures

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Abstract

The 1,9-dimethylmethylene blue dye-binding assay for sulfated glycosaminoglycans has been modified and adapted for use on a Technicon autoanalyzer. The assay was linear for chondroitin sulfate from 2.5 to 500 μg/ml and used only 2.5 μl sample. Interference by salts and solvents commonly used in proteoglycan research was minimal and could be compensated for by using standards in the appropriate solvent. High concentrations of albumin and serum interfered strongly but most of the inhibition could be overcome by papain digestion.

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