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Effect of short-chain fatty acid on sodium absorption in isolated human colon perfused through the vascular bed

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Abstract

A method of perfusing the isolated human colonin vitro was developed to study the effect of the short-chain fatty acidn-butyrate on sodium absorption under controlled conditions. The isolated colon was viablein vitro provided that ischemia to the colon prior to perfusion was less than 40 min. Viability was judged on glucose utilization, mucosal potential difference, and sodium absorption. Sodium absorption from the lumen was observed either with or without 20 mMn-butyrate. In a control group sodium absorption (nmol/min/cm2±sem) was 320±10 (four perfusions, nine observation intervals) and potassium secretion 26±3 (four perfusions, nine observation intervals). With 20 mMn-butyrate sodium absorption was 1960±480 (four perfusions, ten observation intervals) (P<0.0025). Potassium secretion was 72±2 (four perfusions, ten observation intervals) (P<0.025). Butyrate absorption was 254±60 (four perfusions, ten observation intervals) and correlated linearly with the unidirectional flux (J m→s ) of sodium (linear coefficient of 0.714,P=<0.001). These results suggest that the presence of bacterial short-chain fatty acids may determine the efficiency of sodium absorption in the colon and also indicate that an absence of short-chain fatty acids in the colon could be one factor leading to diminished sodium absorption in the colon of man.

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References

  1. McNeil NI, Cummings JH, James WPT: Short-chain fatty acid absorption by the human large intestine. Gut 19:819–822, 1978

    Google Scholar 

  2. Roediger WEW, Truelove SC: Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies. Gut 20:484–488, 1979

    Google Scholar 

  3. Roediger WEW: Functional activity of the colonic mucosa in health and in ulcerative colitis. DPhil thesis, Oxford, 1979

  4. Henning SJ, Hird FJ: Ketogenesis from butyrate and acetate by the caecum and the colon of rabbits. Biochem J 130:785–790, 1972

    Google Scholar 

  5. Robinson JWL, Rausis C, Basset P, Mirkovitch V: Functional and morphological response of the dog colon to ischaemia. Gut 13:775–783, 1972

    Google Scholar 

  6. Robinson JWL, Haroud M Winistorfer B, Mirkovitch V: Recovery of function and structure of dog ileum and colon following two hours of acute ischaemia. Eur J Clin Invest 4:443–452, 1974

    Google Scholar 

  7. Argenzio RA, Miller N, Von Engelhardt W: Effect of volatile fatty acids on water and ion absorption from the goat colon. Am J Physiol 229:997–1002, 1975

    Google Scholar 

  8. Debongie JC, Phillips SF: Capacity of the human colon to absorb fluid. Gastroenterology 74:698–703, 1978

    Google Scholar 

  9. Parsons DS, Powis G: Some properties of a preparation of rat colon perfusedin vitro through the vascular bed. J Physiol (London) 217:641–663, 1971

    Google Scholar 

  10. Krebs HA, Henseleit K: Untersuchungen uber die harnstoffbildung im tierkorper. Hoppe Seylers Z Physiol Chem 210:33–66, 1932

    Google Scholar 

  11. Hems R, Ross BD, Berry MN, Krebs HA: Gluconeogenesis in the perfused rat liver. Biochem J 101:284–293, 1966

    Google Scholar 

  12. Kavin H, Levin NW, Stanley MM: Isolated perfused rat small bowel technic, studies of viability and glucose absorption. J Appl Physiol 22:604–611, 1967

    Google Scholar 

  13. Windmueller HG, Spaeth AE, Ganote CE: Vascular perfusion of the isolated rat gut: Norepinephrine and glucocorticoid requirement. Am J Physiol 218:197–204, 1970

    Google Scholar 

  14. ross BD: Perfusion Techniques in Biochemistry—A Laboratory Manual. Oxford, Clarendon Press, 1972

    Google Scholar 

  15. Rubinstein R, Howard AV, Wrong OW:In vivo dialysis of faeces as a method of stool analysis IV. The organic ion component. Clin Sci 37:549–564, 1969

    Google Scholar 

  16. Natelson S: Routine use of ultramicro methods in the clinical laboratory. Am J Clin Pathol 21:1153–1195, 1951

    Google Scholar 

  17. Hyden S: A turbidimetric method for the determination of higher polyethylene glycols in biological materials. K Lantbruksakad Annaler 22:139–145, 1956

    Google Scholar 

  18. Bergmeyer HU: Methods of Enzymatic Analysis, Vol 3. New York, Academic Press, 1973

    Google Scholar 

  19. Visscher MB, Fetcher ES, Carr CW, Gregor HP, Bushey MS, Barker DE: Isotope tracer studies on the movement of water and ions between intestinal lumen and blood. Am J Physiol 142:550–575, 1944

    Google Scholar 

  20. Edmonds CJ: Electrical potentials of the sigmoid colon and rectum in irritable bowel syndrome and ulcerative colitis. Gut 11:867–874, 1970

    Google Scholar 

  21. Rask-Masden J: The relationship between sodium fluxes and electrical potentials across the normal and inflamed human rectal wallin vivo. Acta Med Scand 194:311–317, 1973

    Google Scholar 

  22. Rask-Masden J: Functional alterations in ulcerative colitis.In Intestinal Ion Transport. JWL Robinson (ed). Lancaster, MTP, 1976, pp 381–397

    Google Scholar 

  23. Postaire JG, Gerard J, Devroede G, Van Houhe AN: Correlation of variations in intraluminal pressure and potential difference in the perfused colon. Gut 18:771–778, 1977

    Google Scholar 

  24. Randle PH, Garland PB, Newsholme EA: Regulations of glucose uptake by muscle. Biochem J 93:652–655, 1964

    Google Scholar 

  25. Hulten L, Jodal M, Lindhagen J, Lundgren O: Blood flow in the small intestine of cat and man as analyzed by an inert gas washout technique. Gastroenterology 70:45–51, 1976

    Google Scholar 

  26. Mirkovitch V, Menge H: Movements of water and ions across dog ileal mucosa following ischaemia or occlusion.In Intestinal Ion Transport. JWL Robinson (ed). Lancaster MTP, 1976, pp 251–268

    Google Scholar 

  27. Levitan R, Fordtran JS, Burrows BA, Ingelfinger FJ: Water and salt absorption in the human colon. J Clin Invest 41:1754–1759, 1962

    Google Scholar 

  28. Harris J, Shields R: Absorption and secretion of water and electrolytes by the intact human colon in diffuse untreated proctocolitis. Gut 11:27–33, 1970

    Google Scholar 

  29. Parsons DS, Paterson CR: Fluid and solute transport across rat colonic mucosa. Q J Exp Physiol 59:220–231, 1965

    Google Scholar 

  30. Parsons dS: Energetics of intestinal transport.In Intestinal Absorption and Malabsorption. TZ Csaky (ed). New York, Raven Press, 1975, pp 9–36

    Google Scholar 

  31. Dawson AM, Holdsworth CD, Webb J: Absorption of short-chain fatty acids in man. Proc Soc Exp Biol Med 117:97–100, 1964

    Google Scholar 

  32. Ruppin H, Bar-Meir S, Soergel KM, Wood C, Schmitt MG: Propionate transport by the human colon. Clin Res 25:609A, 1977

    Google Scholar 

  33. Davenport HW: Physiology of the Digestive Tract, 3rd ed. Year Book Medical Publishers, Chicago, 1977, p 217

    Google Scholar 

  34. Phillips SF: Diarrhoea: A current view of the pathophysiology. Gastroenterology 63:495–518, 1972

    Google Scholar 

  35. Torres-Pinedo R, Lavaslida M, Rivera CK, Rodriguez H, Oritz A: Studies on infant diarrhoea 1. A, comparison of the effects of milk feeding and intravenous therapy upon the composition and volume of the stool and urine. J Clin Invest 45:469–480, 1966

    Google Scholar 

  36. Bustos-Fernandez L, Gonzales E, Ledesma de Paolo I, Celener D, De Furuya KO: Organic anions induce colonic secretion. Am J Dig Dis 21:329–332, 1976

    Google Scholar 

  37. Asatoor AM, Chamberlain MF, Emmerson BT, Johnson JR, Levi AJ, Milne MD: Metabolic effects of oral neomycin. Clin Sci 33:111–124, 1967

    Google Scholar 

  38. Gompertz D, Brooks AP, Gaya M, Spiers ASD: Volatile fatty acids in the faeces of patients in “germ-free” isolation. Gut 14:183–186, 1973

    Google Scholar 

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Roediger, W.E.W., Moore, A. Effect of short-chain fatty acid on sodium absorption in isolated human colon perfused through the vascular bed. Digest Dis Sci 26, 100–106 (1981). https://doi.org/10.1007/BF01312224

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  • DOI: https://doi.org/10.1007/BF01312224

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