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Hyaluronic acid

An indicator of pathological conditions of human lungs?

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Abstract

Hyaluronic acid was the only glycosaminoglycan found in detectable amounts in the pulmonary secretions of patients with cystic fibrosis. The compound gave a hexuronate/hexosamines molar ratio of approximately 1. Glucosamine represented over 98% of the total hexosamines, the remainder being galactosamine. No hexoses or sulfate could be detected. It moved as a single spot with the mobility of standard hyaluronic acid on cellulose acetate electrophoresis and this spot disappeared after digestion with testicular hyaluronidase. It was associated with trace amounts of protein, the major amino acids of which are aspartic acid, glutamic acid, glycine, and alanine.

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References

  1. Wusteman, F. S. 1972. Glycosaminoglycans of bovine lung parenchyma and pleura.Experientia 28:877–888.

    Google Scholar 

  2. Friman, C., P. E. Hellstrom, M. Juvani, andH. Riska. 1977. Acid glycosaminoglycans in the differential diagnosis of pleural effusion.Clin. Chim. Acta 76:357–361.

    Google Scholar 

  3. Havez, R., P. Degand, A. Boersma, andC. Richet. 1971. Characterisation et dosage de l'acide hyaluronique.Clin. Chim. Acta 33:443–454.

    Google Scholar 

  4. Sahu, S., andW. S. Lynn. 1978. Hyaluronic acid in pulmonary secretions of patients with asthma.Biochem. J. 173:565–568.

    Google Scholar 

  5. Sahu, S., andW. S. Lynn. 1978. Hyaluronic acid in pulmonary secretions of patients with alveolar proteinosis.Inflammation 3:149–158.

    Google Scholar 

  6. Kylstra, J. A., D. C. Rousch, D. K. Hall, andA. Spock. 1971. Volume-controlled lung lavage in the treatment of asthma, bronchiectasis and mucoviscidosis.Am. Rev. Resp. Dis. 103:651–665.

    Google Scholar 

  7. Sahu, S., andW. S. Lynn. 1977. Lipid composition of airway secretions from patients with asthma and patients with cystic fibrosis.Am. Rev. Resp. Dis. 115:233–239.

    Google Scholar 

  8. Dische, Z.. 1947. A new specific colour reaction of hexouronic acid.J. Biol. Chem. 167: 189–198.

    Google Scholar 

  9. Bitter, T., andH. Muir. 1962. Modified uronic acid carbazole reaction.Anal. Biochem. 4:330–334.

    Google Scholar 

  10. Swann, D. A., andJ. B. Caulfield. 1975. Studies on hyaluronic acid.Conned, Tissue Res. 4:31–39.

    Google Scholar 

  11. Swann, D. A., andE. A. Balazs. 1966. Determination of hexosamine content of macromolecules.Biochim. Biophys. Acta 130:112–129.

    Google Scholar 

  12. Sahu, S., andW. S. Lynn. 1979. Characterization of high-molecular-weight glycoprotein isolated from pulmonary secretions of patients with alveolar proteinosis.Biochem. J. 177: 153–158.

    Google Scholar 

  13. Spiro, R. G.. 1966. Analysis of sugars found in glycoproteins.Methods Enzymol. 8:3–26.

    Google Scholar 

  14. Sahu, S., R. P. DiAugustine, andW. S. Lynn. 1976. Lipids found in pulmonary lavage of patients with alveolar proteinosis and in rabbit lung lamellar organelles.Am. Rev. Resp. Dis. 114:177–185.

    Google Scholar 

  15. Baker, J. R., J. A. Cifonelli, andL. Roden. 1975. Linkage of corneal keratan sulfate to protein.Connect. Tissue Res. 3:149–156.

    Google Scholar 

  16. Spencer, B. 1960. Ultramicro determination of inorganic sulfate.Biochem. J. 75:435–440.

    Google Scholar 

  17. Oegema, T. R., V. C. Hascall, andD. D. Dziewiatkowski. 1975. Isolation and characterization of proteoglycans from swarm rat chondrosarcoma.J. Biol. Chem. 250:6151–6159.

    Google Scholar 

  18. Swann. D. A., 1968. Studies on hyaluronic acid: Protein components of rooster comb hyaluronic acid.Biochim. Biophys. Acta 160:96–105.

    Google Scholar 

  19. Hamerman, D., M. Rojkind, andJ. Sandson. 1966. Protein bound to hyaluronate: Chemical and immunological studies.Fed. Proc. 25:1040–1045.

    Google Scholar 

  20. Bader, J. P., D. A. Ray, andT. L. Steck. 1972. Electrophoretic determination of hyaluronic acid produced by cells in culture.Biochim. Biophys. Acta 264:73–84.

    Google Scholar 

  21. How, M. J., V. Long, andD. Stanworth. 1969. Association of hyaluronic acid with protein in human synovial fluid.Biochim. Biophys. Acta 194:81–90.

    Google Scholar 

  22. Arai, H., M. Endo, A. Yokosawa, H. Sato, M. Motomiya, andK. Kono. 1975. On acid glycosaminoglycans in pleural effusion.Am. Rev. Resp. Dis. 111:37–42.

    Google Scholar 

  23. Kraemer, P. M.. 1971. Heparan sulfates of cultured cells.Biochemistry 10:1445–1451.

    Google Scholar 

  24. Richards, R. J., andT. G. Morris. 1973. Collagen and mucopolysaccharides production in growing lung fibroblasts exposed to chrysotile asbestos.Life Sci. 12:441–451.

    Google Scholar 

  25. Sampson, P., M. S. Parshley, I. Mandl, andG. M. Turing. 1975. Glycosaminoglycans produced in tissue culture of rat lung cells.Connect. Tissue Res. 4:41–49.

    Google Scholar 

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Author notes

  1. Saura C. Sahu

    Present address: CPSC Health Science laboratory, Room 5860, Federal Building 8, 200 C Street, S.W., 20240, Washington, D.C.

Authors and Affiliations

  1. Departments of Medicine and Biochemistry, Duke University Medical Center, 27710, Durham, North Carolina

    Saura C. Sahu

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  1. Saura C. Sahu
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Sahu, S.C. Hyaluronic acid. Inflammation 4, 107–112 (1980). https://doi.org/10.1007/BF00914107

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  • DOI: https://doi.org/10.1007/BF00914107

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