Monoclonal antibodies used
| Antibody | Specificity | Manufacturer | Working dilution |
|---|---|---|---|
| CD3 | T lymphocytes | DAKO | 1:40 |
| CD4 | T helper/inducer lymphocytes | DAKO | 1:10 |
| CD8 | T cytotoxic lymphocytes | DAKO | 1:50 |
| CD25 | Interleukin 2 receptor, activated T cells | DAKO | 1:40 |
| HLA-DR | MHC type II receptor activated T cells | DAKO | 1:40 |
| Ki67 | Proliferating cell nuclei | DAKO | 1:25 |
| CD138 | Syndecan-1, matrix glycoprotein | Serotec Ltd | 1:100 |
| 6H9 | Eotaxin | See text | 1:200 |
| 2D5 | Monocyte chemotactic peptide 2 | See text | 1:100 |
| Tenascin | Basement membrane component | See text | 1:2000 |