Demonstration of materno-fetal chimerism by HLA typing
| HLA loci1-150 | |||||
|---|---|---|---|---|---|
| A | B | DRB1 | DRB4 | DRB5 | |
| Father (II-17) | 1/11 | 60/51 | 15/4 | 53 | 51 |
| Mother (II-18) | 1/31 | 18/39 | 1/4 | 53 | 51 |
| Patient (III-12) | 1/31/1 | 18/39/51 | 1/15/4 | 53 | 51/51 |
↵1-150 High molecular weight DNA was isolated from lymph node cells (patient) and peripheral blood lymphocytes (both the patient and her parents); HLA typing was performed using PCR-SSP.