Exon | Primer sequence | Annealing temperature | Product (base pairs) |
---|---|---|---|
I-5′ | 5′ CCA CCT GCT GCA GCC TGT CC 3′ | ||
I-3′ | 5′ AGA TGG CAG GGA GGG CGT GG 3′ | 65°C | 183 |
II-5′ | 5′ AGG GTT CAG TGT CAG TGC 3′ | ||
II-3′ | 5′ AAC CCT CAG TGC ACG AAG 3′ | 61°C | 244 |
IIIa-5′ | 5′ GAT GGC AGA CAT GTT TTG 3′ | ||
IIIa-3′ | 5′ GCT GGA TTC AGA CAC CAC 3′ | 58°C | 289 |
IIIb-5′ | 5′ GAG GGT TCA GTG TCA GTG CAG G 3′ | ||
IIIb-3′ | 5′ TCA GAC ACC ACA AAC CAA GAG 3′ | 55°C | 1074 |
IV-5′ | 5′ GTG GGG TGT TGA AAG ATC | ||
IV-3′ | 5′ TGG TAT ATA ACC AGT TCA CAG 3′ | 58°C | 242 |
V-5′ | 5′ TTC TTG CAC TAA AAA AGG TGA T 3′ | ||
V-3′ | 5′ TGT CAC AGC TGA TGC TGG AT 3′ | 58°C | 286 |
VI-5′ | 5′ TTG TGC TTT TGC TAA AAG 3′ | ||
VI-3′ | 5′ ACG ACA CTA TGT CAT CAG 3′ | 55°C | 276 |
VII-5′ | 5′ CTT GGC AAT TTA AAT TGC 3′ | ||
VII-3′ | 5′ TTT CAC AGG AAA GTT CAG 3′ | 61°C | 243 |
VIII-5′ | 5′ GTG GTA TGA TGA TTA TTC 3′ | ||
VIII-3′ | 5′ AAC TAA AGG TGA TCT TAC 3′ | 55°C | 275 |
IXa-5′ | 5′ TGG CAG GCT TTT ATA ATG 3′ | ||
IXa-3′ | 5′ AGG TAC GGA TCC TCT TCC 3′ | 55°C | 230 |
IXb-5′ | 5′ GAG CTG TGC AGA GAA GGC 3′ | ||
IXb-3′ | 5′ TGA AGC CAA CCC ACA CAG 3′ | 61°C | 229 |
IXc-5′ | 5′ GAT ATA AAG ATC ATG GGC 3′ | ||
IXc-3′ | 5′ TCT CAC CAG CTG GAA GGG 3′ | 55°C | 262 |