PCR and sequencing primers
| cDNA No (amino acid) | Primer genomic No | Primer sequence | Annealing temperature | Restriction site | Reference No |
|---|---|---|---|---|---|
| IVS2+1 | 979–997 | GAATGTCCCAAGCCTTTGA | 61°C | −Hph1 | 3 |
| 1316–1335 | AGCTGAAGCAAGAGAATCGC | ||||
| c1226G | 5807–5826 | GCCTTTGCTCTTACCCTCGA | 61°C | +Xho1 | NP |
| (N370S) | 5906–5926 | ACGAAAGTTACGCACCCAATT | |||
| c1342C | 5878–5897 | GCTTGCCCTGAACCCCGAAG | 58°C | −Sty1 | NP |
| (D409H) | 6031–6050 | GAGATGATAGGCCTGGTATG | |||
| c1448C | 5904–5923 | CCAATTGGGTGCGTAACTTT | 61°C | +Nci1 | NP |
| (L444P) | 6655–6691 | CTCACTGGCGACGCCACAGGTAGGTGTGAATGGAGTA | |||
| c1504T | 5904–5923 | CCAATTGGGTGCGTAACTTT | 61°C | −Msp1 | NP |
| (R463C) | 6655–6691 | CTCACTGGCGACGCCACAGGTAGGTGTGAATGGAGTA | |||
| c1604A | 6632–6651 | TCCTGGAGACAATCTCACCT | 61°C | +Hph1 | 3 |
| (R496H) | 6782–6801 | TAAGCTCACACTGGCCCTGC | |||
| c84 G 85ins | N:1007–1036 | N:GCATCATGGCTGGCAGCCTCACAGGATTGC | 70°C | NP | |
| 84GG | M:1007–1036 | M:GCATCATGGCTGGCAGCCTCACAGGATTGG | |||
| ARMS | C:1350–1373 | C:GCGTAGTGGTGGCCGCCTGTAATC | |||
| Exon No | |||||
| 9–11 | 5569–5588 | AACCATGATTCCCTATCTTC | 50°C | 10 | |
| 7314–7333 | GGTTTTTCTACTCTCATGCA | ||||
| 9 | 5797–5816 | CCAGTGTTGAGCCTTTGTCT | 58°C | 10 | |
| 6782–6801 | GAGATGATAGGCCTGGTATG | ||||
| 10 | 6322–6341 | GGTTTCATGGGAGGTACCCC | 58°C | NP | |
| 6542–6561 | GAGAGTGTGATCCTGCCAAG |
NP = new primer.
Genomic nucleotide numbering is according to Horowitz et al.7 cDNA numbering begins with the A of the upstream initiator codon. The reference sequence for the cDNA numbering is M11080.