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Evaluation of salivary anti-Salmonella typhi lipopolysaccharide IgA ELISA for serodiagnosis of typhoid fever in children
  1. Zeeba Zaka-ur-Rab1,
  2. Shaad Abqari2,
  3. Tabassum Shahab2,
  4. Najmul Islam3,
  5. Indu Shukla4
  1. 1Department of Pediatrics, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, India
  2. 2Department of Pediatrics, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, India
  3. 3Department of Biochemistry, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, India
  4. 4Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, India
  1. Correspondence to Dr Zeeba Zaka-ur-Rab, Department of Pediatrics, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002 (UP), India; zzrab{at}yahoo.co.in

Abstract

This observational study was conducted to determine the diagnostic accuracy of ELISA for the detection of anti-Salmonella typhi lipopolysaccharide (LPS) salivary immunoglobulin A (IgA) antibodies in 37 children with culture confirmed typhoid, 30 febrile controls with an alternative diagnosis and 30 healthy controls. The test was positive in 33/37 (89.2%) cases of typhoid, but negative in all patients in the two control groups. Maximum absorbance of anti-LPS IgA was observed during the second and third weeks of typhoid, with a progressive decline thereafter. The sensitivity of ELISA was 71.4%, 100%, 100%, 9.1% and 0%, in first, second, third, fourth and fifth week of illness, respectively. Further large scale studies measuring salivary anti-LPS IgA antibodies are needed to confirm the potential of saliva-based serology in children with suspected typhoid.

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Footnotes

  • Competing interests None.

  • Ethics approval This study was conducted with the approval of the Institutional Ethics Committee, Jawaharlal Nehru Medical College, Faculty of Medicine, A.M.U., Aligarh, India.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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