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Comparison of Interferon-gamma release assays and Tuberculin Skin Test in predicting active tuberculosis (TB) in children in the UK- a Paediatric TB Network Study
  1. Alasdair R J Bamford1,
  2. Crook M Angela2,
  3. Julia Clark3,
  4. Zohreh Nademi3,
  5. Garth Dixon4,
  6. James Y Paton5,
  7. Anna Riddell6,
  8. Francis Drobniewski7,
  9. Andrew Riordan8,
  10. Suzanne T Anderson9,
  11. Amanda Williams10,
  12. Sam Walters11,
  13. Beate Kampmann1,*
  1. 1 Imperial College London, United Kingdom;
  2. 2 MRC Clinical Trials Unit, United Kingdom;
  3. 3 Newcastle General Hospital, United Kingdom;
  4. 4 Great Ormond Street Hospital, United Kingdom;
  5. 5 University of Glasgow, United Kingdom;
  6. 6 The Royal London Hospital, United Kingdom;
  7. 7 HPA Mycobacterium Reference Unit, United Kingdom;
  8. 8 Alder Hey Children's NHS Foundation Trust, United Kingdom;
  9. 9 Brighton and Sussex Medical School, United Kingdom;
  10. 10 Northwick Park Hospital, United Kingdom;
  11. 11 Imperial College Helathcare NHS Trust, United Kingdom
  1. Correspondence to: Beate Kampmann, Paediatrics, Imperial College London, Flat 10, 17 Cadogan Square, London, SW1X 0HT, United Kingdom; b.kampmann{at}imperial.ac.uk

Abstract

Background: The value of interferon-gamma release assays (IGRA) to diagnose active tuberculosis (TB) in children is not established, but these assays are being widely used for this purpose. We examined the sensitivity of commercially available IGRA to diagnose active TB in children in the UK compared with the tuberculin skin test (TST).

Methods: We established a paediatric tuberculosis network (PTBNET-UK) and conducted a retrospective analysis of data from children investigated for active TB at six large UK paediatric centres. All centres had used TST and at least one of the commercially available IGRA (T-Spot.TB or Quantiferon-Gold in Tube) in the diagnostic work up for active TB. Data were available from 333 children aged 2months to 16 years. We measured the sensitivity of TST and IGRA in definite (culture confirmed) and probable TB in children, agreement between TST and either IGRA and their combined sensitivity.

Results: Of 333 children, 49 fulfilled the criteria of definite TB and 146 had probable TB. Within the definite cohort, TST had a sensitivity of 82%, Quantiferon-Gold in tube (QFT-IT) had a sensitivity of 78% and T-Spot.TB of 66%. Neither IGRA performed significantly better than a TST with a cut-off of 15 mm. Combining results of TST and IGRA increased the sensitivity to 96% for TST plus T-Spot.TB and 91% for TST plus QFG-IT in the definite TB cohort.

Conclusions: A negative IGRA does not exclude active TB disease, but a combination of TST and IGRA increases the sensitivity for identifying children with active TB.

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