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O-180 Avidity Of Anti-pertussis Toxin (pt) Igg-antibodies After Primary And Booster Pertussis Vaccination And Their Association With Il-17a Gene Polymorphism
  1. A Barkoff1,
  2. K Gröndahl-Yli-Hannuksela1,
  3. J Vuononvirta1,
  4. V Peltola2,
  5. J Ilonen3,
  6. J Mertsola2,
  7. Q He4
  1. 1Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland
  2. 2Pediatrics, Turku University Hospital, Turku, Finland
  3. 3Medical Microbiology and Immunology, University of Turku, Turku, Finland
  4. 4Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland

Abstract

Background and aims High levels of antibodies and avidity indicates good protection after vaccination. However, there are only a few studies measuring avidity of PT-antibodies in children. Recent studies suggest that Th17 specific immunity acquired from Diphtheria-Tetanus-acellular-Pertussis (DTaP) vaccination may provide efficient protection against pertussis. In this study, we aimed to investigate concentration and avidity of anti-PT-IgG antibodies (PT-Abs) after primary and booster vaccination and their association with gene polymorphism of IL-17A.

Methods Altogether, 325 serum samples were included. From these, 72 were collected from unvaccinated infants at 2.6 months of age, 203 from primary vaccinated 13-month-old children, and 50 from DTaP vaccinated adults. Concentration and avidity of PT-Abs were measured by ELISA. SNP detection of IL-17A was performed using Sequenom iPlex Gold system.

Results Quantity of PT-Abs showed significant increase after primary vaccination in infants. When primary and booster vaccinations were compared, significantly higher levels of PT-Abs were observed after booster vaccination, whereas higher levels of avidity were found after primary vaccination. Frequencies of three IL-17A genotypes identified was 33% (G/G), 47% (G/A) and 20% (A/A) in 203 infants. Subjects with IL-17A G/G genotype had significantly lower avidity of PT-Abs than those with the other two genotypes. However, there was no significant difference in levels of PT-antibodies between these genotypes.

Conclusions Our results indicate that avidity of PT-Abs is higher after primary vaccination than after booster vaccination. This study also suggests that gene polymorphism of IL-17A may influence quality of PT-Abs after primary vaccination in infants.

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