Purpose To explore the role of JNK in the treatment of intestinal trefoil factor for necrotizing enterocolitis in rats
Methods Thirty wistar rats, one day after birth, were equally divided into three groups: normal control group, NEC model group, and NEC+ITF group. The rats were fed for 3 days and killed on the 4th day. The ileocecum were obtained for histological examination by HE staining, JNK protein expression detection by Western-Blot and JNK gene transcripts detection by RT-PCR.
Results The level of JNK protein expression and gene transcripts in NEC model group was higher than that in normal control group. Compared with NEC Model group, the level of JNK expression and gene transcripts was obviously decreased in NEC+ITF group.
Conclusion The JNK signalling transduction pathway may play important roles in the pathogenesis of NEC. ITF may protect the mucosa from injury by inhibiting JNK signalling transduction pathway.
- Necrotizing enterocolitis
- Intestinal trefoil factor