Introduction Decreased platelet number and/or function have been related to patent ductus arterious (DA). Activated platelets release vasoactive products, including serotonin (5-HT) that might be relevant for DA homeostasis DA. The chicken embryo has emerged as a suitable model for the study of DA vascular biology. In the present study, we investigated the possible vasoactive role of 5-HT in the chicken DA.
Methods Rings of the DA of 15- to 20-d-old chicken embryos (total incubation time 21-d) were studied in a wire myograph. The response to 5-HT was investigated under different O2 tensions (3, 7, and 74 kPa). The responses to the 5-HT1B/D receptor agonist sumatriptan, the 5-HT2A/B/C receptor agonist DOI and the selective serotonin reuptake inhibitors fluoxetine and sertraline were also investigated.
Results 5-HT (10 nM-0.3 mM) contracted the pulmonary side of the DA (PulmDA) in a concentration-dependent manner. By contrast, 5-HT induced negligible contractions in the vessels that surround the PulmDA (i.e., the pre- and post-ductal pulmonary arteries, and the aortic side of the DA). 5-HT-induced contraction increased with development (15-d >17-d >19-d=20-d). O2 tension did not affect 5-HT-induced contraction but elimination of extracellular calcium completely abolished it. Sumatriptan and DOI also contracted the PulmDA in a concentration-dependent manner. By contrast, fluoxetine and sertraline evoked contractions at very high concentrations (>0.1 mM).
Conclusions Our data indicate that 5-HT receptors are functionally present in the chicken DA and suggest that platelet-derived 5-HT may play a pivotal role in the postnatal closure of the DA.
Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.