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PS-301 Conditions Of Intestinal Colonisation In Preterm Infants During The First Month Of Life
  1. E Martín Alvarez,
  2. L Zamorano-Bonilla,
  3. MV Jiménez-Cabanillas,
  4. L Serrano-López,
  5. M Peña-Caballero,
  6. JA Hurtado-Suazo
  1. Paediatric, Hospital Virgen de Las Nieves, Granada, Spain

Abstract

Aim To describe intestinal microbiota in preterm infants admitted to the Neonatal Unit using molecular techniques, and assess the impact of different conditions.

Methods/study design A two year period Descriptive study (2011–2012) of gut microbiota in stools from newborns borns under 35 week gestational age (WGA) at birth, admitted in a Neonatal Care Unit. Stool samples were collected: M1 (meconium), M2 (first week), M3 (first month). 5 groups of bacteria were analysed using qPCR technique: Bacteroides, Bifidobacterium, Escherichia coli, Clostridiums and Lactobacillus. Maternal, perinatal and neonate variables were registered. Statistic programs SPSSvs 20.

Abstract PS-301 Table 1

ResultsIn the first month a marked increase (x120) in Bifidobacterium is observed. The increase of Lactobacillus (x2) and E. coli (x7) is lower. Bacteroides and Clostridium remain stable.

Regarding WGA in ≤ 30 vs > 30 we observed:

- Bifidobacterial colonisation is delayed at birth (2,35 × 104 vs 6,02 × 104 cfu/g; p = 0,09) and at one week birth age (3,98 × 104 vs 1,49 × 106 cfu/g; p = 0,007).

- Higher numbers of E. coli from the first sample (4,37 × 103 vs 9,07 × 102 cfu/g; 1,55 × 104 vs 2,93 × 103 cfu/g; 2,94 × 104 vs 9,69 × 103 cfu/g), although no significant statistically differences were detected.

Conclusions The number of Bifidobacterium and Lactobacillus in faecal samples is higher than the content in Bacteroides, Clostridium and E.coli. The colonisation process of studied bacteria is delayed in prematures born at lower WGA, except for E. coli.

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