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PS-159 Beta-amyloid And S100b Could Be Valuable Biomarkers After Neonatal Hypoxia-reoxygenation. A Study In Newborn Pigs
  1. T Benterud1,
  2. L Pankratov1,
  3. R Solberg1,
  4. OD Saugstad1,
  5. A Skinningsrud2,
  6. N Bolstad3
  1. 1Department of Pediatric Research, Oslo University Hospital, Oslo, Norway
  2. 2Department of Medical Biochemistry, Akershus University Hospital, Oslo, Norway
  3. 3Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway

Abstract

Background and aims The use of biomarkers is an important strategy for risk stratification for neonates admitted to the NICU after perinatal asphyxia. Total Tau (t-Tau), phosphorylatedTau (p-Tau) and Beta-Amyloid (1–42) (b-A) in CSF, are useful biomarkers in various neurological disorders, such as Alzheimer's. Few, if any, experiments have investigated the possible association between oxidative stress in the neonates and the levels of these proteins.

Objective To study any correlations between the levels of t-Tau, p-Tau, b-A, S100B and NSE and oxidative stress in the newborn pig.

Methods Fifty-four newborn piglets, age12–36 h, were included. One control group (n = 6) and 2 experimental groups (n = 24), exposed to global hypoxia (8% O2) until BE reached -15 mmol/l (moderate hypoxia) or -20 mmol/l (severe hypoxia) or BP fell below 20 mmHg. The pigs were observed for 9.5 h. CSF was collected 9.5 h after the intervention.

Discussion To our knowledge this is the first study investigating the levels of Tau, p-Tau and b-Amyloid in a neonatal animal model after hypoxia-reoxygenation. The reduced level of CSF b-Amyloid may reflect the death of neurons after neonatal hypoxia. A strategy of measuring several biomarkers should be applied when assessing the prognosisafter perinatal asphyxia.

Conclusion These findings show that b-amyloid and S100b are significantly changed in neonatal pigs subjected to hypoxia compared to controls, thus they may be valuable biomarkers after perinatal asphyxia.

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