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O-203b Paediatric Microdose Study Of [14c]paracetamol To Study Drug Metabolism Using Accelerated Mass Spectrometry: Proof Of Concept
  1. MG Mooij1,
  2. E van Duijn2,
  3. CA Knibbe3,
  4. W Vaes2,
  5. B Fabriek2,
  6. AD Windhorst4,
  7. NH Hendrikse4,
  8. LM Hanff5,
  9. BCP Koch5,
  10. PJJ M. Janssen5,
  11. D Tibboel1,
  12. S de Wildt1
  1. 1Department of Pediatric Surgery and Intensive Care, Erasmus University Medical Center – Sophia Children’s Hospital, Rotterdam, Netherlands
  2. 2Innovation for Life, TNO, Zeist, Netherlands
  3. 3Clinical Pharmacology, Leiden Academic Center for Drug Research, Leiden, Netherlands
  4. 4Nuclear Medicine and PET Research, VU Medical Center, Amsterdam, Netherlands
  5. 5Clinical Pharmacy, Erasmus MC, Rotterdam, Netherlands

Abstract

Rationale Microdosing is a promising new method to obtain pharmacokinetic data in children with minimal burden and minimal risk. The use of a labelled oral microdose offers the added benefit to study intestinal and hepatic drug disposition in children already receiving an intravenous therapeutic drug dose for clinical reasons.

Objective To present pilot data of an oral [14C]paracetamol (AAP) microdosing study as proof of concept for this method to study developmental pharmacokinetics in children.

Methods In an open microdose pharmacokinetic pilot study, infants (0–6 yrs of age) received a single oral [14C]AAP microdose (3.3 ng/kg, 60 Bq/kg) in addition to intravenous therapeutic doses of AAP (15 mg/kg IV q6 h) prescribed by the treating physician to provide analgesia. Blood samples were taken from an indwelling catheter at multiple time points. AAP blood levels were measured by LC-MS/MS and [14C]AAP and metabolites ([14C]AAP-Glu and [14C]AAP-4Sul) were measured by accelerator mass spectrometry.

Results Ten infants (ranging from 0.1 to 83.1 months of age) were included, one patient was excluded from PK analysis, as he vomited shortly after administration. In all 9 patients, [14C]AAP and metabolites in blood samples were detectable at expected concentrations. Dose normalised [14C]AAP Cmax concentrations approached median Cav intravenous concentrations: median 8.41 mg/L (range 3.75 to 23.78 mg/L) and 8.87 mg/L (range 3.45 to 12.9 mg/L), respectively.

Conclusions We demonstrate the practical and ethical feasibility to use a [14C]labelled microdose to study paracetamol pharmacokinetics, including metabolite disposition, in young children.

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