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186 Protective Effects of Colchicine in an Experimental Model of Necrotizing Enterocolitis in Neonatal Rat
  1. S Yurttutan1,
  2. R Ozdemir1,
  3. FE Canpolat1,
  4. MY Oncel1,
  5. HG Unverdi2,
  6. B Uysal3,
  7. O Erdeve1,
  8. U Dilmen1
  1. 1Neonatal Intensive Care Unit, Zekai Tahir Burak Maternity Teaching Hospital
  2. 2Department of Pathology, Ankara Etlik Ihtisas Training and Research Hospital
  3. 3Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey

Abstract

Introduction The pathophysiology of necrotizing enterocolitis (NEC) includes massive production of endogenous cytokines with exaggerated activation of inflammatory pathways. Colchicine has been used as an anti-inflammatory agent. The aim of this study was to investigate the possible beneficial effects of colchicine in a neonatal rat model of NEC.

Methods Rats were randomly divided into 3 groups: control group; saline-treated NECgroup; colchicine-treated NEC group. NEC was induced by hyperosmolar enteral formula feeding and exposure to hypoxia/reoxygenation after cold stress. Intestinal samples were harvested for biochemical and histopathological analysis.

Results The grade of intestinal injury of pups in the saline-treated NEC group was found to be significantly higher than in the control or the colchicine-treated groups (p<0.001, p=0.003; respectively). Median level of intestinal malondialdehyde was significantly higher in the saline-treated NEC group compared to the control group (p=0.006) and the colchicine-treated group (p=0.015). Significantly higher activities of intestinal superoxide dismutase and glutathione peroxidase activities were observed in the colchicine-treated NEC group compared to the saline-treated group (p=0.033 and p=0.030; respectively). Tissue levels of tumor necrosis factor- α and interleukin 1β were significantly higher in the saline-treated NEC group compared to rats in the colchicine-treated group (p<0.001, p=0.003; respectively). A comparison of saline-treated and colchicine-treated NEC pups revealed that treatment with colchicine was associated with significantly lower tissue levels of TNF- α and IL-1β (p<0.01, both).

Conclusion We observed that; in this model of NEC, colchicine has favorable effects onintestinal histological and biochemical changes.

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