Background The watershed of Dez and Karoon rivers located in middle Zagrous mountain with area about 68481-, Thus watershed is a part of Persian gulf watershed. Cholera, an acute intestinal infection caused by the bacterium Vibrio cholerae (V. cholerae) is a historically feared epidemic diarrheal disease that remains a major public health problem in many parts of Africa, Asia, and Latin America. V. cholerae O1 exists as two major serotypes, Inaba and Ogawa, a member of the family Vibrionaceae is transmitted through Fresh water contaminated with fecal matter. Foodborne infections have been traced to raw. The target of study the Segregate V. cholerae 01 (Vibrionaceae) in the Karoon Ahvaz River.
Methods In four stages (April, to July 2010), a total 100 samples of water from Karoon River Ahvaz were collected. During the study period the recorded river temperature was about 25–28°C and pH ranged from 7 to 8. Swabs were cultured onto thiosulphate citrate bile sucrose and MacConkey, and morphological colonies compatible with Vibrio were characterized by oxidase test and agglutinated with antiserum for serotype determination. Also V. cholerae biochemical tests with API 20E.
Results From 100 samples of water Karoon River in Ahvaz, Iran, 8 (8%) sample were positive for Vibrio cholerae strains. The isolated strains from water Karoon River in Ahvaz, Iran, were Vibrio cholerae O1 (inaba).
Conclusion The priorities for cholera control remain public health interventions through improved water and sanitation, improved surveillance and access to health care facilities, and further development of appropriate vaccines.