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921 Virulence Factors of Uropathogenic and Commensal Escherichia Coli Pathotypes in Patients with Urinary Tract Infection
  1. M Navidinia1,
  2. S Najar Peerayeh1,
  3. F Fallah2,
  4. B Bakhshi1,
  5. S Adabian2,
  6. Z Gholinejad2,
  7. S Alimehr2,
  8. MA Malekan2
  1. 1Tarbiat Modares University
  2. 2Shahid Beheshti University of Medical Sciences, Mofid Children Hospital, PIRC, Tehran, Iran

Abstract

Background The present study is comparison of commensal and uropathogenic Escherichia coli (UPEC) pathotypes according to multiple virulence genes by multiplex PCR.

Methods Thirty two virulence factors including adhesions group, protectin related genes, common toxins related to UPEC, phylogenic typing, Pathogenicity Island and miscellaneous genes which are involved in Escherichia coli pathogenicity mechanisms were examined by PCR.

Results The PCR assay results identified vat(96 vs. 4%), PAI IV536(85 vs. 41%), PAI ICFT073(78 vs. 25%), kpsMTII(71 vs. 42%), ompT(68 vs. 53%),usp(52 vs. 3%), sat(47 vs. 3%), picU (41 vs. 1%), PAI IICFT073(38 vs. 13%), PAI I536(37 vs. 7%), fliC (H7(24 vs. 9%)), hlyD(22 vs. 2%), focG(21 vs. 3%), cnf1(21 vs.0%), gafD(20 vs. 2%), cdtB (19 vs. 7%),bmaE(17 vs. 5%), PAI II J96(11 vs.3%), were more frequent virulence markers in urinary E.coli isolates than commensal E.coli, respectively. The frequency of afa(7 vs.10%), fimH(92 vs. 97%), kpsMT(K1) (41 vs. 57%),kpsMTIII(11 vs.5%), rfc (O4 LPS) (9 vs. 2%), PAI II536(20 vs. 24%), PAI III536(3 vs.0%), PAI I J96( 53 vs. 43%), ibeA(17 vs. 26%) markers was almost similar in both of them. cvaC(64 vs. 18%)was most frequent marker in commensal E.coli. PCR phylotyping revealed higher prevalence of commensal E. coli in groups A and D while uropathogenic strains were mainly found in subgroup B2.

Conclusion Based on these results, the potential for Commensal E.coli to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered.

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