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888 Antibiotics Study on Staphylococcus Spp. Strains Isolated from Venous and Urinary Catheters in Nicu of Hamadan Hospitals, West Ran
  1. R Yousefimashouf1,
  2. N Molazadeh2
  1. 1Department of Microbiology, Research Center for Molecular Medicine of Hamadan University of Medical Sciences
  2. 2Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract

Background and Aim Staphylococcus coagulase negative strains are colonized on epiderm and distribute in environment and outer bodies apparatus such as protez and intera-venous catheters. The aim of this study was the frequency of Staphylococcus coagulase negative isolated from venous and catheters children hospitalized in NICU of Hamadan hospitals and determination of antibiotics resistance patterns in Hamadan, the west of Iran .

Methods We collected 108 samples randomly from patients who were hospitalized in NICU hospitals of Hamadan and they needed to venous or urinary catheters. One specimen of each patient was taken and inoculated into carrier transported media and transferred to bacteriology laboratory to identification of strains. Antibiogram was performed by Kirby-Bauer method. Data was analyzed using SPSS 15 software.

Results Out of 108 tested samples, 32.7% of patients had urinary catheter and 67.3% had venous catheter. 28% of tested samples had positive culture. The positive cases were significantly found in those children who had been used catheter more than 48 hours (P = 0.00). From the positive cases, Staphylococcus epidemidis (40.4%), Acinetobacter baumannii (10.6%) and E. coli (8.5%) were the most common isolates. The most rate of resistance of Staphylococcus epidemidis was against to erythromycin and ampicillin. The most rate of sensitivty of E. coli was against to gentamicin and Psuedomonas aeruginosa to ciprofloxacin.

Conclusion Our results showed the high contamination in used catheters particularly in those patients who needed to catheter for long time. We also indicated the high drug resistance in strains isolated from catheters.

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