Background Probiotic bacterial lectins are important factors in biotope. The aim was to study autoregulation properties of lectins of lactobacilli (LL) isolated by us .
Methods Clinical strains of lactobacilli were freshly isolated from urogenital tract samples of young and adolescent patients using growth on Lactobacillus MRS Agar (HiMedia) and variant of Shaedler medium. Additional differential indication media were used. Bacterial growth was studied in the presence of disc- or drop- applied of LL on solid media or LL within bacterial suspensions (0.5–5 McFarland units). Samples studied were characterized with lactobacilli at the level of 106–108 Cfu/ml, and did not contain a panel of standard bacterial and viral pathogens. Decreased contents of Staphylococcus ssp., Enterococcus ssp., E. coli, and Candida ssp. were registered.
Results All methods used gave similar results (supporting each other) of action of LL on lactobacilli populations growth. The following groups of lactobacilli populations were identified: LL-resistant or sensitive, weakly suppressive or stimulated, highly or moderate suppressive, highly modulated, cell condensation depended or not.
Conclusions Results indicate further evidences that probiotic LL imitate cellular probiotics. It seems LL are involved in signal events (QS, cross-talking) in biotope. LL may act as a biotope tool of microbiota selection and may be used for both supporting probiotic-like lactobacilli and simultaneous elimination of other lactobacilli possessing decreased survival (non-probiotic-like strains) in conditions of healthy or close to normal balance in biotope.
Reference . V. Lakhtin, M. Lakhtin, V. Pospelova, B. Shenderov (2006) Microb. Ecol. Health & Dis. 18: 55–60.