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728 DNA Damage and Apoptosis Evaluation in Leukocytes of Icteric Newborns after Phototherapy Treatment by Using of the Neutral Comet Assay
  1. M Shahidi1,
  2. SA Mesbah-Namin2,
  3. S Heidari3
  1. 1Department of Biochemistry and Biophysics Mazandaran University of Medical Sciences, Sari
  2. 2Department of Biochemistry, Tarbiat Modarres University, Tehran
  3. 3MSC, Molecular Genetics, Saint Mary Fertility Center, Sari, Iran

Abstract

Background and Aims Phototherapy is the most common form of treatment for jaundice. one of the various endpoints that can be used to investigate the potential of blood as a predictor of radiosensitivity is DNA damage and apoptosis. The aim of this study was to investigate the possible relation between phototherapy and DNA damage and apoptosis, in neonates with hyperbilirubinemia.

Methods The study included 15 healthy full-term newborns as control. The phototherapy group consisted of 30 non-physiologic jaundice that after 16 hours phototherapy, 1 ml peripheral venous blood were obtained of them.

Results DNA damage immediately after phototherapy termination (IPT) was higher in jaundice infants than control one (p<0.01). After 24 hours cells repaired their damages, as there wasn’t any difference between these groups about this value. DNA damage in the phototherapy group was higher at IPT than 24 hours after that, but in the control one there weren’t any differences between them(respectively p<0.001, p>0.05). Apoptosis value at 24 hours after phototherapy termination was higher than the IPT in the phototherapy group and was statistically significant (P<0.001).

Conclusions Since repair proofreading and fidelity properties isn’t absolutely, then always this is probable that errors maybe occur during extensive DNA damages repair and finally these errors can cause mutation in DNA. If this event be in important and sensitive region of genome, harmful effects would menace phototherapy-treated infants’ later-life. So this is necessary to investigation of long-term genotoxic effects of phototherapy in phototherapy-treated neonates.

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