Background Probiotic human bacterial mixtures constructed are important for health [1]. The aim was to develop new perspective variants of the known Russian mixed probiotic “Acilact” (Lactobacillus strains NK1+K₃III₂₄+100_ash; all strains of human origin) using approach of ranging new parameters of cultural fluids, their fractions and components.

Methods The system of at least 32 parameters was ordered as decreased/increased sequences of 4 members (Acilact and its strains) depending on any selected parameter. The cases of leadership (inverted or not in the sequence) of Acilact or strain(s) are considered as technological advantages of consortium or strain(s).

Results and discussion Among high molecular mass components (at least 30 kD), protein fractions, lectin systems, protease system, oxidase-reductase system, biosurfactants, intrinsic fluorofores containing protein and non-protein components, and activities against eukaryotic human pathogens were evaluated as perspective new features and criteria for construction of new system probiotics of differentially directed action. Non-linear proportional contributions of ingredient strains into final properties of Acilact were observed. Strains as dominant leaders in some features revealed in Acilact can be detected. Examples of significantly new advantages of ingredient strain(s) or Acilact are presented. The net of ordered ranges allows additional control of the results.

Conclusion The approach developed can help in constructing qualitatively new effective and directed mixed extended probiotics based on traditionaliy used probiotic bacterial industrial strains.

Reference [1]. V.V. Pospelova. Thesis (MD) [Microbial biological preparations for correction of intestinal bacteriocenoses, their designing and using (in Russian)]. Moscow. 1979.