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DIFFERENTIAL VIRAL REPLICATION FOLLOWING IN-VITRO RESPIRATORY SYNCYTIAL VIRUS INFECTION OF MATCHED PAEDIATRIC PRIMARY BRONCHIAL AND NASAL EPITHELIAL CELLS

1D. O’Donoghue, 2G. Skibinski, 2L. Heaney, 3U. Power, 2M. Shields. 1Royal Belfast Hospital for Sick Children, Belfast, UK, 2Respiratory Research Group, Queen’s University, Belfast, UK, 3Department of Immunobiology, Queen’s University, Belfast, UK

Background: Respiratory syncytial virus (RSV) is the most commonly isolated virus causing acute bronchiolitis. It typically presents with rhinorrhea, followed in a proportion of children by lower respiratory tract signs. An in-vitro study demonstrated no difference in infectivity to rhinovirus in upper and lower epithelial cells.1 Viral replication following RSV infection has not previously been studied in matched paediatric primary bronchial epithelial cells (PBEC) and primary nasal epithelial cells (PNEC).

Aims: To compare viral replication following in-vitro infection of matched paediatric PBEC and PNEC.

Methods: PBEC and PNEC were obtained from paediatric subjects by non-bronchoscopic bronchial and nasal brushings following the induction of anaesthesia for elective surgical procedures. The matched PBEC and PNEC were cultured to passage three and were infected with a wild-type RSV A at a multiplicity of infection of 0.1 and also with mock infection. Cell lysates were collected in duplicate at regular post-infection time points up to 96 h post-infection and were used to measure viral replication. Viral replication was determined by serial dilutions to look for evidence of cytopathic effect and expressed as log 50% tissue culture infective doses (TCID50)/ml. Repeated measures analysis of variance was used for statistical analysis of viral replication over the postinfection time course.

Results: Matched PBEC and PNEC were obtained and cultured from six paediatric subjects with a median age of 5.2 years (range 2–8 years). PNEC supported significantly greater wild-type RSV A replication than PBEC (p<0.01) over the experimental time course.

G85 Figure

Mean carbon dioxide (CO2) in each baby during weaning.

Conclusion: There is greater viral replication …

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