Article Text

TH1/TH2 CYTOKINE PROFILES IN SUBTYPES OF JUVENILE IDIOPATHIC ARTHRITIS AND JUVENILE SYSTEMIC LUPUS ERYTHEMATOSUS
  1. V N Umlauf1,
  2. M Kirchner1,
  3. P Schmidtke1,
  4. W Mannhardt-Laakmann1
  1. 1Pediatric Immunology and Rheumatology Department, Center for Pediatric and Adolescent Medicine, Hospital of the Johannes Gutenberg-University, Mainz, Germany

Abstract

Objective Although the cause of juvenile idiopathic arthritis (JIA), a heterogeneous group of childhood arthritides and systemic lupus erythematosus (SLE) remains unknown, it is well known that cytokines are the main mediators of inflammation. Th1 cytokines, such as IL-1, IL-2, IL-8, IL-12, tumour necrosis factor (TNF)-α, and (IFN)-γ, bear proinflammatory functions, whereas Th2 cytokines such as IL-4, IL-5, and IL-10 support anti-inflammatory tasks. Th1-produced IL-6 acts as both a pro and anti-inflammatory cytokine. This study’s aim is to investigate how immunological profiles differ in JIA subgroups and SLE compared with healthy controls by measuring Th1/Th2 cytokine serum levels.

Methods 150 children (29 systemic JIA, 38 persistent oligoarthritis, 15 seronegative polyarthritis (SNP), 12 enthesitis-associated arthritis (EAA), 24 SLE, 32 healthy controls) were included in this study. A multiplex fluorescent bead immunoassay was used for quantititative detection by flow cytometry of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IFN-γ, and TNF-α in whole blood.

Results Leading proinflammatory cytokines in systemic JIA and oligoarthritis are IL-12 and IL-6, their anti-inflammatory antagonist is IL-4. Outstanding cytokines in SNP are proinflammatory TNF-α and IL-1β with their opponent IL-5. EAA is associated with the pro-/anti-inflammatory match IL-1β and IL-2/IL-10, whereas SLE is associated with INF-γ/IL-4. IL-8 was not significantly increased in the diseases.

Conclusions In this study we demonstrated different cytokine profiles with leading pro and anti-inflammatory cytokines in different autoinflammatory diseases. Some profiles might be useful for determinating the JIA subtype. Furthermore, the leading proinflammatory cytokines may be targets for new therapy strategies in future.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.