Article Text

DETERMINATION OF D/L-ARABINITOL RATIO, PRESENCE OF FUNGAL DNA AND PROCALCITONIN LEVELS AS MARKERS FOR CANDIDIASIS IN PREMATURE INFANTS
  1. T J Stradomska1,
  2. M K Kornacka2,
  3. U Majewska2,
  4. B Garczewska3,
  5. D Dzierżanowska3
  1. 1Department of Laboratory Diagnostics, The Children’s Memorial Health Institute, Warsaw, Poland,
  2. 2Neonatal Department, Warsaw Medical University, Warsaw, Poland,
  3. 3Department of Clinical Microbiology and Immunology, The Children’s Memorial Health Institute, Warsaw, Poland

Abstract

It is considered that premature infants in invasive of methods treatment condition are a considerable risk group for fungal infection, particularly for opportunistic microorganisms (C. albicans constitutes 50–80%), due to immune system immaturity. The essential problem of the efficacious treatment for candidiasis is lack of sensitive and fast diagnostic methods.

d-arabinitol, a characteristic metabolite of several Candida sp. is applied as an alternative non-invasive, diagnostics biomarker for candidiasis.

The study group (A) encompassed 51 premature infants, born 24–36 Hbd, suspected of fungal infection (mothers had vaginal fungal infections).

The control group had: (C1), 49 premature infants (born 26–30 Hbd) with birth weights, mean±SD = 1036.74±298.56 g and (C2), 45 premature infants (born 31–35 Hbd) with birth weights, mean±SD = 1904.18±405.67 g.

Urinary d/l-arabinitol ratio was determined by GC-ECD methods. Molecular analysis for presence of fungal DNA was made by PCR. Serum procalcitonin levels was performed with LUMI test PCT.

In the control groups C1 and C2, d/l-arabinitol ratio was, mean±SD = 2.14±0.54 and 2.16±0.53 respectively. The cut off value was d/l-arabinitol = 3.8.

In the study group A there were applied methods (d/l-arabinitol, PCR, procalcitonin) but non-confirmed fungal infection (vs control group p>0.5). In 49 infants d/l-arabinitol ratio, procalcitonin levels were at normal values, results of molecular analysis of DNA did not indicate the presence of Candida sp. Two children with superficial colonization (C. albicans) had a d/l-arabinitol ratio in range of the cut off value limit.

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