Article Text

MESENCHYMAL STEM CELLS IN TREATMENT OF GROWTH PLATE AND ARTICULAR CARTILAGE
  1. L Planka1,
  2. D Stary1,
  3. A Necas2,
  4. R Srnec2,
  5. J Hlucilova3,
  6. L Kren1,
  7. P Gal1
  1. 1Child Surgery, Orthopedic and Traumatology Clinic, Faculty Hospital Brno, Brno, Czech Republic
  2. 2Department of Surgery and Orthopaedics, Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
  3. 3Institute of Animal Physiology and Genetics of The Academy of Sciences of The Czech Republic, Libechov, Czech Republic

Abstract

Objective Assorted treatment methods for growth plate and articular cartilage injury in children are known. The using of MSCs (mesenchymal stem cells) from bone marrow blood brings new possibilities in the treatment of these defects.

Methods Group A was represented by 10 pigs; allogenic MSCs were transplanted into a iatrogenic defect on the lateral side of distal epiphysis of left femur. Group B was represented by 30 pigs; MSCs were transplanted into a circular osteochondral defect (0.6 mm). In group A all femurs were measured for the length of bone and valgus deformity. The final results in both groups were made by histological examination (H-E, Pearls, PAS, ELISA, FISH).

Results In group A the left femurs were on average 0.56±0.44 cm longer than the right femurs. Average angular deformity of the left femur was 0.78, while on the right femur without transplantation 3.7°. In groups A (left femurs) and B we can see the creation of hyalin cartilage in most of the experimental animals. In group A (right femurs) a bone bridge in the iatrogenic partial growth plate arrest was created.

Conclusion MSCs transplantation into the growth plate defect can hide the creation of valgous deformity and restrict the length of growth failure of the bone. Also, the MSC transplantation into the articular cartilage defect leads to much better healing than the common methods. This work was supported by grants from Research Projects of IGA MZ CR NR9296-2/2007 and MSMT (NPV II 2B06130).

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