Article Text

EFFECT OF NICOTINE AND RESUSCITATION WITH 21% OR 100% O2 ON NF-KB IN NEWBORN HYPOXIC TRANSGENIC LUCIFERASE REPORTER MICE
  1. J Alsharnoubi1,
  2. H H Odland1,
  3. H Carlsen2,
  4. R Blomhoff2,
  5. O D Saugstad1
  1. 1Department of Paediatric Research, Rikshospitalet Medical Center, Oslo, Norway
  2. 2Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

Abstract

Alsharnoubi et al

Objective Resuscitation with 100% compared with 21% O2 increases inflammatory responses in the lungs and the brain. We have previously shown that the transcription factor NF-κB is elevated in adult hypoxic mice resuscitated with 100% O2.

Methods Seven-day-old transgenic mice expressing luciferase as reporter of NF-κB activity were divided into two groups, which were either injected (n = 140) or not injected (n = 148) with nicotine subcutaneously, 2.4 mg/kg of nicotine base dissolved in 9% saline. They were then exposed to 40 min FiO2 of 0.08 and further randomized to be resuscitated with either 21% or 100% O2 for 30 min. Light produced by the luciferase reaction which reflects NF-κB activity were detected by the IVIS Imaging system 100 series. The whole body (Total) and the brain area were measured. NF-κB activity at maximal velocity of the reaction are reported.

Results There were no effects of nicotine (Nic) on the groups. Mean and SD for NF-κB activities are given (see table).

Conclusions Nicotine in the doses used in this study had no effect on NF-κB activity. Resuscitation with 100% O2 reduced NF-κB when comparing with use of 21% O2. This is the opposite result to what we have previously reported regarding adult mice.

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