Article Text

CORRELATION ANALYSIS BETWEEN AQUAPORIN-1,-2,-3,-4 MRNA EXPRESSION AND RENAL PARENCHYMA THICKNESS IN CONGENITAL HYDRONEPHROTIC KIDNEY IN CHILDREN
  1. J G Wen1,2,
  2. Z Z Li1,2,
  3. Y Z Fan1,2,
  4. Q Zhang1,2,
  5. H Zhang2,3
  1. 1Urodynamic Center, Department of Pediatric Surgery, First Teaching Hospital of Zhengzhou University, Zhengzhou, China
  2. 2Institute of Clinical Medicine Henan, First Teaching Hospital of Zhengzhou University, Zhengzhou, China
  3. 3Department of Pathology, First Teaching Hospital of Zhengzhou University, Zhengzhou, China

Abstract

Objective To investigate the relationship between aquaporin -1,-2,-3,-4 mRNA (AQP1-4) and renal parenchyma thickness in congenital hydronephrotic kidney in children.

Methods The expression of aquaporin 1,-2,-3, and -4 mRNA in hydronephrotic kidney of 37 children (aged 60.28±48.8 months) were evaluated with congenital hydronephrosis and normal kidney of 6 children (aged 62.67±17.06 months) using semi-quantitative reverse transcriptase polymerase chain reaction technique. Hydronephrotic kidney parenchyma thickness were measured by B-Ultrasound preoperatively and verified at operation. The relation of aquaporin 1,-2,-3, and -4 mRNA to the hydronephrotic kidney parenchyma thickness were analyzed by linear regression.

Results The aquaporin 1,-2,-3, and -4/beta-actin ratio in the hydronephrotic kidney and normal kidney were AQP1:0.39±0.22 VS 0.90±0.10; AQP2:0.42±0.20 VS 0.92±0.09; AQP3: 0.52±0.22 VS 0.98±0.12; AQP4: 0.30±0.18 VS 0.74±0.21, respectively, and the differences were significant (p<0.01). Hydronephrotic kidney parenchyma thickness measured by B-Ultrasound is averaged at 5.01±2.38 mm, which is identical with those measured at operation. Significant correlation was found between the levels of aquaporin 1,-2,-3,and -4 mRNA and hydronephrotic kidney parenchyma thickness (r = 0.773, 0.772, 0.557, 0.625, respectively; p<0.01).

Conclusions Significant correlation exists between decreased expressions of aquaporin 1,-2,-3, and -4 mRNA and atrophic change of renal parenchyma, providing evidence to explain the mechanism why the thinner renal parenchyma thickness, the weaker renal concentration and dilution function.

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