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STUDY OF THE EXPRESSION OF ADHESION MOLECULES IN AN EXPERIMENTAL MODEL OF HYPOXIC ISCHEMIC ENCEPHALOPATHY IN FETAL LAMBS
  1. D Alonso-Alconada1,
  2. F J Alvarez2,
  3. A Alvarez1,
  4. F Goni-de-Cerio1,
  5. E Gastiasoro2,
  6. M C Rey-Santano2,
  7. V E Mielgo2,
  8. J Lacalle1,
  9. A Valls-i-Soler2,3,
  10. E Hilario1
  1. 1Department of Cell Biology and Histology, School of Medicine, University of The Basque Country, Leioa, Vizcaya, Spain
  2. 2Research Unit On Pediatrics, Hospital de Cruces, Osakidetza, Barakaldo, Vizcaya, Spain
  3. 3Department of Pediatrics, School of Medicine, University of The Basque Country, Leioa, Vizcaya, Spain

Abstract

Objective To evaluate the expression levels of inter cellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), Integrin-α4β1 and fibronectin after hypoxic-ischemic brain injury induced by partial occlusion of the umbilical cord (PCO) in fetal lambs.

Methods Nine fetal lambs at 86–92% gestation were randomly assigned to three experimental groups: one healthy group (sacrificed 3 h after delivery) and two hypoxic-ischemic groups: one sacrificed immediately after HI event (HI-0h) and the other sacrificed 3 h after HI event (HI-3h). Injury was induced by PCO during 60 min. Brains were perfusion-fixed and divided into different cerebral regions which were disaggregated and immunolabelled for ICAM-1, VCAM-1, Integrin-α4β1 and fibronectin. Cell suspensions were analyzed by flow cytometry. ANOVA (p<0.05).

Results HI-0h group showed a decrease in the percentage of positive cells (p<0.05), in comparison to healthy ones, in all regions studied for Integrin-α4β1 and fibronectin (except in the hippocampus). With respect to VCAM-1 and ICAM-1, a statistically significant decrease was only found in the cortex. Three hours after injury, Integrin-α4β1 maintained low levels in comparison to the healthy group in all brain regions, whereas this was only true of fibronectin in the cortex, cerebellum and thalamus. VCAM-1 and ICAM-1 differ in the cortex and cerebellum. In the comparison of both HI groups, both Integrin-α4β1 and fibronectin showed an increase in cortex and medulla oblongata, after 3 h, whereas VCAM-1 decreased (p<0.05) in all brain regions (except hippocampus).

Conclusion Our data suggest a decrease in the expression of adhesion molecules just after HI brain injury.

Grants: “Fundación Jesús-Gangoiti-Barrera”, UPV/EHU06/99, Basque-Government (IT-287-07) and FIS06/0908.

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