Article Text

  1. J-M Hascoet1,
  2. C Hubert1,
  3. C Boschat3,
  4. F Rochat3,
  5. H Legagneur2,
  6. S Emady-Azar3,
  7. S Gaga1,
  8. P Steenhout4
  1. 1Neonatology Department, Maternite Regionale Universitaire, Nancy, France
  2. 2Department of Biology, Maternite Regionale Universitaire, Nancy, France
  3. 3Nestle Research Center, Lausanne, Switzerland
  4. 4Nestle Nutrition, Nestec Ltd., Vevey, Switzerland


Objective To assess the effect on microbiotia development of a standard control formula, a formula with a composition close to human milk, Nan, and investigate the equivalence between Nan and Nan + a probiotic, Bifidobacterium longum (BL999; Morinaga, Japan).

Methods Randomized, double blind, controlled trial of 3 groups: control formula; Nan : 30/70 Casein/Whey ratio (vs.70/30), lower protein (1.8 vs.2.5 g/100 Kcal); lower phosphate (31 vs.70 mg/100 Kcal) and higher lactose levels (11.1 vs. 4.5 g/100 Kcal); Nan + BL999 (2×107 cfu/g) and a reference BF group. Healthy singleton term infants received the study formulae for 4 months. Primary outcome was stool bifidobacteria counts (log CFU/g: Median (IQR)) measured by FISH method at 2 months of age.

Results Bifidobacteria counts were significantly higher for BF (10(0.9), p<0.0001, n = 35; 49.7% of total bacteria (TB)), or Nan (10.0(0.8), p<0.0001 n = 33; 52.6% TB) or Nan +BL999 (9.8(1.4), p<0.01 n = 33; 35.3% TB) groups than controls (9.2(3.5), n = 33; 31.9%TB). The same profile between study formulae was observed with Enterobacteria (8.5, 8.4, 8.1% vs. 13.1% TB respectively) or Clostridium (5.6, 9.4, 7.5 vs. 18.9% TB respectively).

Conclusions These data show that infant formula with a composition closer to human milk (with or without BL999) is more bifidogenic, and leading to a closer intestinal microbiotia profile to BF, than a standard formula.

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