Article Text

EFFECT OF NICOTINE AND RESUSCITATION WITH 21% OR 100% OXYGEN ON NUCLEAR FACTOR KAPPA B IN NEWBORN HYPOXIC TRANSGENIC LUCIFERASE REPORTER MICE
  1. J Alsharnoubi1,
  2. H H Odland1,
  3. H Carlsen2,
  4. R Blomhoff2,
  5. O D Saugstad1
  1. 1Department of Paediatric Research, Rikshospitalet, University of Oslo, Oslo, Norway
  2. 2Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

Abstract

Objective Resuscitation with 100% compared with 21% oxygen increases inflammatory responses in the lungs and the brain. We have previously shown that the transcription factor nuclear factor kappa B (NF-κB) is elevated in adult hypoxic mice resuscitated with 100% oxygen.

Methods Seven-day-old transgenic mice expressing luciferase as a reporter of NF-κB activity were divided into two groups either injected or not with nicotine subcutaneously, 2.4 mg/kg of nicotine base dissolved in 0.9% saline. They were then exposed to 40 minutes FiO2 of 0.08. Random selection was carried out both to compare 30-minute resuscitation with either room air or 100% oxygen and also to study any effects of nicotine. Light produced by the luciferase reaction, which reflects NF-κB activity, was detected by the IVIS imaging system 100 series. The whole body and the brain area were measured and NF-κB activity at maximal velocity of the reaction is reported.

Results There were no effects of nicotine on the groups. There were no differences in NF-κB activity either in the whole body or the brain area in animals resuscitated with 21% or 100% oxygen.

Conclusion Nicotine in the doses used in this study had no effect on NF-κB activity. By contrast to previous studies with adult mice in which NF-κB was significantly elevated after resuscitation with 100% compared with 21% oxygen, no difference between the groups was found in the present study.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.