BK virus DNA in CSF of immunocompetent and immunocompromised patients
- 1Professor Alborzi Clinical Microbiology Research Center, Clinical Virology Section, Shiraz University of Medical Sciences, Shiraz, Iran
- 2Virology, Public Health Laboratory, Leeds, UK
- 3Division of Virology, Laboratory Medicine Academic Group, University of Manchester, 3rd Floor, Clinical Sciences Building, Manchester Royal Infirmary, UK
- Correspondence to:
Dr A Behzad-Behbahani, Professor Alborzi Clinical Microbiology Research Center, Virology Section, Namazee Hospital, Shiraz, Iran;
- Accepted 24 July 2002
Aim: To investigate the possible aetiological role of BK and JC viruses in immunocompetent and immunocompromised children with suspected encephalitis and meningoencephalitis.
Methods: The polymerase chain reaction and microplate hybridisation method was employed for the detection of polyomavirus DNA in 266 CSF specimens collected from immunocompetent and immunocompromised patients.
Results: BK virus DNA was detected in three (2.1%) CSF samples taken from patients aged 2–5 years; two were patients with acute lymphocytic leukaemia without overt neurological symptoms, the other was a patient with suspected encephalitis. BK virus DNA was also detected in two (1.6%) CSF samples taken from older children in the age range 10–16 years; both children had suspected encephalitis. JC virus DNA was not found in any CSF sample from either age group.
Conclusions: Detection of BK virus in the CSF of immunocompromised and immunocompetent patients with suspected neurological disease suggests that this virus may have had a pathogenic role in the aetiology of this condition.
- BKV, BK virus
- CNS, central nervous system
- CSF, cerebrospinal fluid
- JCV, JC virus
- PCR, polymerase chain reaction
- RT, reverse transcriptase