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Sex hormone binding globulin concentration as a prepubertal marker for hyperinsulinaemia in obesity

Abstract

BACKGROUND Overweight children become obese adults who are prone to develop the “metabolic syndrome” and premature coronary arterial disease (CAD).

AIMS To assess whether sex hormone binding globulin (SHBG) is a potential marker for hyperinsulinaemia/insulin resistance in prepubertal obese children.

METHODS Twenty five obese children (body mass index (BMI) >2SD) who warranted investigation on clinical grounds were enrolled. Their insulin response to an oral glucose tolerance test was assessed.

RESULTS Fourteen children were hyperinsulinaemic. Despite being matched for age and BMI, SHBG concentrations were below the sex related reference range in the hyperinsulinaemic group.

CONCLUSION Our results indicate that a subnormal SHBG concentration in a prepubertal child is strongly predictive of hyperinsulinaemia. By measuring the circulating SHBG concentration, it might be possible to identify those at most risk of premature CAD, targeting them for lifestyle changes.

  • SHBG
  • insulin
  • prepubertal
  • obesity

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The current trend of increasing childhood obesity in the industrialised world continues unabated.1-3 Childhood obesity carries with it the burden of significant health consequences in adult life, as illustrated by a Finnish study showing that the risk of suffering from the metabolic syndrome (hypertension, hyperinsulinaemia, hyperlipidaemia, and visceral obesity) in adulthood was increased 56-fold (CI: 13 to 504) if obesity began during childhood4 (obesity defined as top tertile). Furthermore it has been shown that there is an increased incidence of obesity in children whose parents had early coronary artery disease (CAD).5

Obese children most at risk are likely to be those who have inherited the polygenic propensity for hyperinsulinaemia/insulin resistance considered by many to be the central aetiological factor for the metabolic syndrome.6 The ability to target such children, and their families, for exercise and nutritional education could reduce their risk of developing the metabolic syndrome and premature CAD. To date, biochemical assessment has relied on prolonged dynamic testing using either the insulin response to a glucose load or by euglycaemic clamp, procedures that are not appropriate for screening children in an obesity clinic.

In addition to androgens and thyroxine, insulin is known to regulate sex hormone binding globulin (SHBG) production by the liver.7 Low circulating SHBG concentrations are known to occur in adults as a consequence of hyperinsulinaemia/insulin resistance.8 We wished to investigate whether this might also be the case in prepubertal children such that it could provide the basis of a screening test for childhood hyperinsulinaemia.

Patients and methods

We enrolled 25 prepubertal obese children for oral glucose tolerance testing (OGTT). We defined obese children as those whose body mass index (BMI) was greater than 2 SD from the mean.9 All received 45 g/m2 oral glucose at 9 am, after fasting from 10 pm the preceding night. Venous blood samples were collected before the glucose load and at 30 minute intervals for two hours. Plasma was rapidly separated and stored at −20°C; insulin was measured by an in house radioimmunoassay with a coefficient of variation of 10% at 15 pmol/l. Using the trapezoid rule, the area under the insulin/time curve (AUC) was calculated. We considered values >100 pmol/l/2 h diagnostic of insulin resistance as derived locally from adult euglycaemic clamp studies.10

We recruited 57 healthy schoolchildren (27 boys, 30 girls; aged 5–10 years), with no evidence of pubertal development, and 71 older healthy children (38 boys, 33 girls; aged 10–17 years), for whom pubertal status was not recorded, as a reference paediatric population. At least four samples were obtained from children of each gender for each year of age. All had a venous blood sample collected and analysed for SHBG by immunoradiometric assay (Orion Diagnostica kit supplied by Pharmacia Biosystems Ltd, Milton Keynes) with an interassay coefficient of variation of 7%.

The Yorkhill ethical committee approved this study. Informed and written consent was obtained from the parents of all children who underwent detailed clinical and biochemical investigation.

STATISTICAL ANALYSIS

Comparison between the hyperinsulinaemic and non-hyperinsulinaemic children involved unpaired t tests on the age and BMI SD scores, and the Mann–Whitney U test on SHBG concentrations. Data were analysed with Minitab statistical software (Minitab data analysis software, release 8.1, standard version; Minitab Inc., 3081 Enterprise Drive, State College, PA 16801, USA).

Results

In the normal prepubertal healthy schoolchildren group, circulating SHBG ranged from 35 to 168 nmol/l in boys and 44 to 143 nmol/l in girls (mean ± 2SD). These values decreased with increasing age, approaching adult concentrations (6–45 nmol/l in boys and 30–120 nmol/l in girls) by 17 years.

The 25 children who underwent oral glucose tolerance tests had no physical signs of sexual development before testing or on subsequent review four months later. Table 1 shows age, BMI, and SHBG ranges. While the mean age was 7.9 years, the oldest prepubertal child was aged 13 years. Fourteen (10 girls, four boys) of the 25 obese subjects showed an exaggerated insulin response to oral glucose load (AUC >100 pmol/l/2 h) consistent with hyperinsulinism. There was no significant difference in age or BMI between the hyperinsulinaemic and non-hyperinsulinaemic groups. Circulating SHBG concentrations were subnormal in all but two patients in the hyperinsulinaemic group (fig1). Both patients were girls, one of whom had borderline hyperinsulinaemia (AUC 108 pmol/l/2 h). By contrast all but two of the non-hyperinsulinaemic group showed normal SHBG concentrations. Both exceptions were girls with SHBG values of 19 and 36 nmol/l.

Table 1

Characteristics of the 25 obese children studied

Figure 1

Insulin response to a glucose load (45 g/m2) relative to SHBC concentration.

Log transformation showed serum SHBG concentrations were negatively correlated with OGTT insulin AUC (r = −0.65). In our group of obese prepubertal children (n = 25), a circulating SHBG concentration below reference range was 82% specific in predicting an exaggerated OGTT insulin response with 86% sensitivity.

Discussion

Our data support the hypothesis that, as has been shown in adults,8 the circulating SHBG concentration in childhood and adolescence is a marker for hyperinsulinaemia/insulin resistance. Circulating SHBG concentrations in our normal population group showed a decrease as puberty approaches, which was more notable in boys. This decrease coincides with the well documented phase of insulin resistance associated with puberty.11

We have shown a strong association between low SHBG concentrations with documented hyperinsulinaemia in obese children. None of the children showed evidence of early or precocious puberty during or shortly after the study, and the insulin resistance appeared to be a consequence of being overweight.

Although obesity is clearly a cause of insulin resistance, other factors must be responsible as only 56% of our obese study group was hyperinsulinaemic. Moreover, the actual degree of obesity in hyperinsulinaemic and non-hyperinsulinaemic children was similar.

Our finding that the circulating SHBG concentration is a better positive predictor of insulin resistance than BMI may facilitate identification of at risk prepubertal children who could be targeted for weight management and lifestyle changes in the hope of reducing their metabolic consequences in later life.

Acknowledgments

We are extremely grateful to Drs MRH Taylor and CV Holland, Department of Paediatrics, Trinity College, Dublin, and Dr PM Crofton, Royal Hospital for Sick Children, Edinburgh, for assistance in obtaining samples from normal children.

References

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