Six children presented during one year with clinical features of infectious mononucleosis, but with laboratory findings of leucocytosis with neutrophilia, increased erythrocyte sedimentation rate, and hypergammaglobulinaemia. Serology for Epstein–Barr virus, cytomegalovirus, adenovirus, and Toxoplasma gondii was negative, while anti-Bartonella henselae IgM with high IgG titre (⩾1/1024) was present in all. All children had contact with kittens. No specific treatment was administered and all recovered.
- Bartonella henselae
- infectious mononucleosis
- cat scratch disease
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Infectious mononucleosis like illness is a multiaetiological clinical syndrome characterised by notable cervical and/or submandibular lymphadenopathy, pharyngotonsillitis, and commonly fever and splenomegaly. Cytomegalovirus (CMV) is the most frequent aetiological agent; less frequently Toxoplasma gondii, adenovirus, rubella, HIV, and herpes viruses may be involved.1 Single cases of rare aetiology caused by drugs or unusual viruses have also been reported, but a number of cases remain undiagnosed.
We observed, over one year, six children with clinical features mimicking infectious mononucleosis in whom we were able to showBartonella henselae infection by appropriate serology.
During one year, all children presenting at the paediatric department of the University of Pisa with clinical features suggesting infectious mononucleosis were investigated for Epstein–Barr virus (EBV), CMV, T gondii, and adenovirus by commercially available serological assays. Serum samples were also tested for the presence of IgG and IgM class antibodies againstB henselae by indirect immunofluorescence antibody test (BIOS GmbH, München, Germany) as previously described.2 An indirect antibody titre of 1/256 or above in the presence of specific IgM was considered indicative ofB henselae infection.3
Table 1 details the principal clinical manifestations and laboratory findings of the six patients. All were outpatients and were in good general condition despite high (>39°C) and prolonged (range 5–35 days) fever. All had bilateral cervical and/or submandibular adenomegaly. The lymph nodes were painless, not inflamed, and exhibited a firm consistency. Other clinical features included slightly erythematous pharyngitis, with tonsillitis without membrane formation and with exudate in three patients; periorbital oedema; nasal obstruction; and splenomegaly in five patients.
Laboratory findings showed: leucocytosis (15.5–28.5 × 109/l) with neutrophilia, thrombocytosis (⩾400 × 109/l), and increased erythrocyte sedimentation rate (ESR; range 54–129 mm/h) in all patients. C reactive protein was increased to up to 12 times the upper limit of normal in all but one. All had hypergammaglobulinaemia; in particular, IgM levels of up to 0.78 g/l were present, and IgG levels were increased, sometimes notably (3.82 g/l). Serum aminotransferase activity was normal in all as was ultrasound examination of the liver and the spleen, performed in five of the six patients. Serology for EBV-VCA IgG and IgM, CMV IgM, adenovirus, and Toxoplasma gondii was negative in all. Anti-B henselae IgM was present in all with an anti-B henselae IgG titre of 1/1024 or above. All children had a history of contact with kittens.
Antibiotic treatment with amoxicillin, ceftriaxone, or clarithromycin had been previously prescribed in five children; after diagnosis no specific treatment was administered and all patients recovered over two to eight weeks.
In the same period of time 62 children affected by EBV related infectious mononucleosis and 14 children with B henselae infection but with different clinical presentations were observed in our paediatric department.
The spectrum of recognised clinical manifestations ofB henselae infection in children has changed a great deal in recent years, with an increasing incidence of atypical presentations such as fever of unknown origin, hepatosplenic granulomatosis, pseudomalignant disorders, or acute encephalopathy.4
Even though some authors suggest that cat scratch disease should be included in the differential diagnosis of all lymphadenopathies,6 infectious mononucleosis like illness has not been specifically reported, even in a recent review,3 as a specific feature suggestive ofB henselae infection.
In our experience the clinical feature of infectious mononucleosis is a presentation of B henselae infection. Clinical features are non-specific: patients are usually younger than 5 years, and present with persistent fever, pharyngitis, tonsillitis with or without exudate, nasal obstruction, periorbital oedema, painless bilateral cervical and submandibular lymphadenitis, and splenomegaly. However, surprisingly neutrophilia rather than lymphomonocytosis, normally typical of both infectious mononucleosis and infectious mononucleosis like illness, characterises the blood smear of these patients. For this reason we suggest the term “pseudoinfectious mononucleosis” to identify this type of onset of cat scratch disease. Other laboratory features supporting the diagnosis include increased ESR and C reactive protein, and hypergammaglobulinemia which may be notable.
The history of contact with a kitten may be a useful diagnostic feature and was present in all our patients.
Serology for B henselae is a sensitive diagnostic procedure.3 High IgG (⩾1/1024) by serum immunofluorescence antibody test and detectable specific IgM were present in all cases. False positive IgM serology forB henselae has been reported during acute EBV infection7; however, all our patients had negative EBV IgG and IgM serology. In cases of suspected “pseudoinfectious mononucleosis”, the significance of anti-B henselae specific IgM should be reconfirmed by demonstration of negative EBV serology and by the presence of significant titres of anti-B henselae IgG.
The value of treatment of B henselaeinfection in the immunocompetent host is debated; most of our patients had been previously treated with different oral antibiotics without evident success. However, as in most forms of B henselae infection in children, “pseudoinfectious mononucleosis” in our patients was followed by complete recovery.